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Literature summary for 1.14.15.15 extracted from

  • Pettersson, H.; Norlin, M.; Andersson, U.; Pikuleva, I.; Bjoerkhem, I.; Misharin, A.; Wikvall, K.
    Metabolism of a novel side chain modified DELTA8(14)-15-ketosterol, a potential cholesterol lowering drug: 28-hydroxylation by CYP27A1 (2008), Biochim. Biophys. Acta, 1781, 383-390.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.15.15

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0047
-
 3beta-hydroxy-5alpha-cholest-8(14)-en-15-one pH 7.4, 37°C Homo sapiens
0.017
-
 (24S)-3beta-hydroxy-24-methyl-5alpha-cholesta-8(14),22-dien-15-one pH 7.4, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(24S)-3beta-hydroxy-24-methyl-5alpha-cholesta-8(14),22-dien-15-one + reduced adrenodoxin + H+ + O2 Homo sapiens the substrate is a potential drug for lowering cholesterol in the treatment and/or prevention of coronary artery disease, but it is rapidly metabolized in the liver ?
-
 ?
3beta-hydroxy-5alpha-cholest-8(14)-en-15-one + reduced adrenodoxin + H+ + O2 Homo sapiens the substrate is a potential drug for lowering cholesterol in the treatment and/or prevention of coronary artery disease, but it is rapidly metabolized in the liver ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-
Hep-G2 cell
-
 Homo sapiens
-
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(24S)-3beta-hydroxy-24-methyl-5alpha-cholesta-8(14),22-dien-15-one + reduced adrenodoxin + H+ + O2 the substrate is a potential drug for lowering cholesterol in the treatment and/or prevention of coronary artery disease, but it is rapidly metabolized in the liver Homo sapiens ?
-
 ?
(24S)-3beta-hydroxy-24-methyl-5alpha-cholesta-8(14),22-dien-15-one + reduced adrenodoxin + H+ + O2 28-hydroxylation by CYP27A1, enzyme activity assay in presence of adrenodoxin, adrenodoxin reductase and NADPH Homo sapiens ? massspectrometric product analysis, 2 products, overview ?
3beta-hydroxy-5alpha-cholest-8(14)-en-15-one + reduced adrenodoxin + H+ + O2 the substrate is a potential drug for lowering cholesterol in the treatment and/or prevention of coronary artery disease, but it is rapidly metabolized in the liver Homo sapiens ?
-
 ?
3beta-hydroxy-5alpha-cholest-8(14)-en-15-one + reduced adrenodoxin + H+ + O2 28-hydroxylation by CYP27A1, enzyme activity assay in presence of adrenodoxin, adrenodoxin reductase and NADPH Homo sapiens ? massspectrometric product analysis, 2 products, overview ?

Synonyms

Synonyms Comment Organism
CYP27A1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0183
-
 (24S)-3beta-hydroxy-24-methyl-5alpha-cholesta-8(14),22-dien-15-one pH 7.4, 37°C, kcat for the conversion of (24S)-3beta-hydroxy-methyl-5alpha-cholesta-8(14),22-dien-15-one into side chain oxygenated products Homo sapiens
0.073
-
 3beta-hydroxy-5alpha-cholest-8(14)-en-15-one pH 7.4, 37°C, kcat for the conversion of (24S)-3beta-hydroxy-methyl-5alpha-cholesta-8(14),22-dien-15-one into side chain oxygenated products Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.001
-
 3beta-hydroxy-5alpha-cholest-8(14)-en-15-one pH 7.4, 37°C Homo sapiens
0.0157
-
 (24S)-3beta-hydroxy-24-methyl-5alpha-cholesta-8(14),22-dien-15-one pH 7.4, 37°C Homo sapiens