Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Gold(DE3) and Rosetta 2(DE3) cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(1R,3S,5Z,7E,14x)-17-[(2R)-1-(1H-imidazol-1-yl)propan-2-yl]-2-methylidene-9,10-secoestra-5,7-diene-1,3-diol | potent and selective CYP24A1 inhibitor | Homo sapiens | |
(1R,3S,5Z,7E,14x)-17-[(2S)-7-cyclopropylheptan-2-yl]-2-methylidene-9,10-secoestra-5,7-diene-1,3-diol | potent and selective CYP24A1 inhibitor | Homo sapiens | |
(2R)-2-[(4E,7aR)-4-[(2Z)-2-[(5R)-5-hydroxy-2-methylidenecyclohexylidene]ethylidene]-7a-methyloctahydro-1H-inden-1-yl]propyl bromoacetate | - |
Homo sapiens | |
(3R,5Z,7E,14x,17x,20S)-3-hydroxy-9,10-secochola-5,7,10-trien-24-yl 4-methylbenzenesulfonate | - |
Homo sapiens | |
24-carboxyl-25-hydroxyvitamin D3 | - |
Homo sapiens | |
24-difluoro-1,25-dihydroxyvitamin D3 | - |
Homo sapiens | |
ketoconazole | nonspecific CYP24A1 inhibitor | Homo sapiens | |
liarozole | nonspecific CYP24A1 inhibitor | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00014 | - |
2-methylene-19-nor-(20S)-1,25-dihydroxyvitamin D3 | in 20 mM Tris (pH 7.5), at 37°C | Homo sapiens | |
0.00019 | - |
1alpha,25-dihydroxyvitamin D3 | in 20 mM Tris (pH 7.5), at 37°C | Homo sapiens | |
0.00033 | - |
25-hydroxyvitamin D3 | in 20 mM Tris (pH 7.5), at 37°C | Homo sapiens | |
0.00034 | - |
2-methylene-19-nor-(20R)-1,25-dihydroxyvitamin D3 | in 20 mM Tris (pH 7.5), at 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
about 55000 Da, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon metal affinity column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2 | - |
Homo sapiens | 1alpha,24R,25-trihydroxyvitamin D3 + NADP+ + H2O | - |
? | |
2-methylene-19-nor-(20R)-1,25-dihydroxyvitamin D3 + NADPH + H+ + O2 | - |
Homo sapiens | ? + NADP+ + H2O | - |
? | |
2-methylene-19-nor-(20S)-1,25-dihydroxyvitamin D3 + NADPH + H+ + O2 | - |
Homo sapiens | ? + NADP+ + H2O | - |
? | |
25-hydroxyvitamin D3 + NADPH + H+ + O2 | - |
Homo sapiens | 24R,25-dihydroxyvitamin D3 + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1alpha,25-dihydroxyvitamin D3 24-hydroxylase | - |
Homo sapiens |
CYP24A1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
2-methylene-19-nor-(20S)-1,25-dihydroxyvitamin D3 | in 20 mM Tris (pH 7.5), at 37°C | Homo sapiens | |
0.0017 | - |
2-methylene-19-nor-(20R)-1,25-dihydroxyvitamin D3 | in 20 mM Tris (pH 7.5), at 37°C | Homo sapiens | |
0.002 | - |
1alpha,25-dihydroxyvitamin D3 | in 20 mM Tris (pH 7.5), at 37°C | Homo sapiens | |
0.002 | - |
25-hydroxyvitamin D3 | in 20 mM Tris (pH 7.5), at 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | CYP24A1 has a heme content of 77-85% | Homo sapiens | |
NADPH | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
2-methylene-19-nor-(20S)-1,25-dihydroxyvitamin D3 | in 20 mM Tris (pH 7.5), at 37°C | Homo sapiens | |
4.9 | - |
2-methylene-19-nor-(20R)-1,25-dihydroxyvitamin D3 | in 20 mM Tris (pH 7.5), at 37°C | Homo sapiens | |
6.1 | - |
25-hydroxyvitamin D3 | in 20 mM Tris (pH 7.5), at 37°C | Homo sapiens | |
10 | - |
1alpha,25-dihydroxyvitamin D3 | in 20 mM Tris (pH 7.5), at 37°C | Homo sapiens |