Cloned (Comment) | Organism |
---|---|
expressed in chinese hamster lung fibroblast cells (V79-4 cells) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A326G | the mutant converts 1alpha-hydroxyvitamin-D3 into 1alpha,25-dihydroxyvitamin-D3-26,23-lactone | Homo sapiens |
V391L | the mutation converts the enzyme from a catabolic 1alpha,25-dihydroxyvitamin-D3-24-hydroxylase into an anabolic 1alpha-hydroxyvitamin-D3-25-hydroxylase. The mutant enzyme retains its basal ability to catabolize 1alpha,25-dihydroxyvitamin D3 via C24 hydroxylation | Homo sapiens |
V391L/A326G | the mutant enzyme continues to form 1alpha,25-dihydroxyvitamin D3 from 1alpha-hydroxyvitamin-D3, but this initial product is diverted via the C23 hydroxylation pathway into 1alpha,25-dihydroxyvitamin-D3-26,23-lactone | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2 | Homo sapiens | - |
calcitroic acid + NADP+ + ? | - |
? | |
25-hydroxyvitamin D3 + NADPH + H+ + O2 | Homo sapiens | - |
24,25-dihydroxyvitamin D3 + NADP+ + H2O | - |
? | |
additional information | Homo sapiens | no activity with 1alpha-hydroxyvitamin D3 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2 | - |
Homo sapiens | calcitroic acid + NADP+ + ? | - |
? | |
25-hydroxyvitamin D3 + NADPH + H+ + O2 | - |
Homo sapiens | 24,25-dihydroxyvitamin D3 + NADP+ + H2O | - |
? | |
additional information | no activity with 1alpha-hydroxyvitamin D3 | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP24A1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |