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Literature summary for 1.14.15.18 extracted from

  • Pedersen, J.I.; Ghazarian, J.G.; Orme-Johnson, N.R.; DeLuca, H.F.
    Isolation of chick renal mitochondrial ferredoxin active in the 25-hydroxyvitamin D3-1alpha-hydroxylase system (1976), J. Biol. Chem., 251, 3933-3941.
    View publication on PubMed
Search for more literature for 1.14.15.18

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Gallus gallus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 kidney mitochondrial 25-hydroxyvitamin D3-1alpha-hydroxylation system consists of a renal ferredoxin reductase (flavoprotein), renal ferredoxin and cytochrome P-450 Gallus gallus
12500
-
 ferredoxin component, gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of ferredoxin component Gallus gallus

Reaction

Reaction Comment Organism Reaction ID
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O kidney mitochondrial 25-hydroxyvitamin D3-1alpha-hydroxylation system consists of a renal ferredoxin reductase (flavoprotein), renal ferredoxin and cytochrome P-450 Gallus gallus
a
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O reconstituted with cytochrome P-450, adrenal ferredoxin, i.e. adrenodoxin, and adrenodoxin reductase Gallus gallus
a
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O ferredoxin, ferredoxin reductase and cytochrome P-450 Gallus gallus
a

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.602
-
 reduction of cytochrome P-450 at non-saturating level of enzyme components Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
calcidiol + NADPH + O2 highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol Gallus gallus calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Gallus gallus