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Literature summary for 1.14.15.18 extracted from
- Enzymatic studies on the key enzymes of vitamin D metabolism; 1alpha-hydroxylase (CYP27B1) and 24-hydroxylase (CYP24) (2001), Biotechnol. Annu. Rev., 7, 179-194.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| vitamin D3 | - |
Homo sapiens |  |
| vitamin D3 | - |
Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
(24R),25-dihydroxycholecalciferol | - |
Homo sapiens | |
| 0.0013 | - |
24,25-dihydroxycholecalciferol | - |
Mus musculus | |
| 0.0027 | - |
25-hydroxycholecalciferol | - |
Mus musculus | |
| 0.0027 | - |
25-hydroxycholecalciferol | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Mus musculus | 5739 | - |
| mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
recombinant enzyme | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (24R),25-dihydroxycholecalciferol + NADPH + O2 | Mus musculus | - |
1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O | - |
? | |
| (24R),25-dihydroxycholecalciferol + NADPH + O2 | Homo sapiens | - |
1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O | - |
? | |
| 25-hydroxycholecalciferol + NADPH + O2 | Mus musculus | recombinant in E. coli | calcitriol + NADP+ + H2O | - |
? | |
| 25-hydroxycholecalciferol + NADPH + O2 | Homo sapiens | recombinant in E. coli | calcitriol + NADP+ + H2O | - |
? | |
| 25-hydroxycholecalciferol + NADPH + O2 | Mus musculus | central role in calcium regulation | calcitriol + NADP+ + H2O | - |
? | |
| 25-hydroxycholecalciferol + NADPH + O2 | Homo sapiens | central role in calcium regulation | calcitriol + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O | reconstitution of the enzyme system in E. coli cells, consisting of NADH-adrenodoxin reductase, adrenodoxin and CYP27B1 | Mus musculus | |
| calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O | reconstitution of the enzyme system in E. coli cells, consisting of NADH-adrenodoxin reductase, adrenodoxin and CYP27B1 | Homo sapiens | |
| calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O | cytochrome P-450 enzyme | Mus musculus | |
| calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O | cytochrome P-450 enzyme | Homo sapiens | |
| calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O | reconstituted with cytochrome P-450, adrenal ferredoxin, i.e. adrenodoxin, and adrenodoxin reductase | Mus musculus | |
| calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O | reconstituted with cytochrome P-450, adrenal ferredoxin, i.e. adrenodoxin, and adrenodoxin reductase | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Mus musculus | - |
| kidney | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000002 | - |
- |
Homo sapiens |
| 0.0000002 | - |
substrate 23,25-dihydroxycholecalciferol | Mus musculus |
| 0.00000042 | - |
substrate 24-oxo-25-hydroxycholecalciferol, recombinant reconstituted system | Homo sapiens |
| 0.00000058 | - |
substrate 25-hydroxycholecalciferol, recombinant reconstituted system | Homo sapiens |
| 0.0000011 | - |
substrate 24-oxo-25-hydroxycholecalciferol | Mus musculus |
| 0.0000015 | - |
- |
Mus musculus |
| 0.0000015 | - |
substrate 24,25-dihydroxycholecalciferol | Homo sapiens |
| 0.0000043 | - |
substrate 24,25-dihydroxycholecalciferol | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (24R),25-dihydroxycholecalciferol + NADPH + O2 | - |
Mus musculus | 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O | - |
? | |
| (24R),25-dihydroxycholecalciferol + NADPH + O2 | - |
Homo sapiens | 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O | - |
? | |
| (24R),25-dihydroxycholecalciferol + NADPH + O2 | higher activity than with 25-hydroxycholecalciferol | Mus musculus | 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O | - |
? | |
| (24R),25-dihydroxycholecalciferol + NADPH + O2 | higher activity than with 25-hydroxycholecalciferol | Homo sapiens | 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O | - |
? | |
| 24,25-dihydroxycholecalciferol + reduced adrenodoxin + O2 | - |
Mus musculus | 1alpha,24,25-trihydroxycholecalciferol + oxidized adrenodoxin + H2O | - |
r | |
| 25-hydroxycholecalciferol + NADPH + O2 | recombinant in E. coli | Mus musculus | calcitriol + NADP+ + H2O | - |
? | |
| 25-hydroxycholecalciferol + NADPH + O2 | recombinant in E. coli | Homo sapiens | calcitriol + NADP+ + H2O | - |
? | |
| 25-hydroxycholecalciferol + NADPH + O2 | central role in calcium regulation | Mus musculus | calcitriol + NADP+ + H2O | - |
? | |
| 25-hydroxycholecalciferol + NADPH + O2 | central role in calcium regulation | Homo sapiens | calcitriol + NADP+ + H2O | - |
? | |
| calcidiol + NADPH + O2 | highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol | Mus musculus | calcitriol + NADP+ + H2O | calcitriol is identical with 1,25-dihydroxycholecalciferol | ? | |
| calcidiol + NADPH + O2 | highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol | Homo sapiens | calcitriol + NADP+ + H2O | calcitriol is identical with 1,25-dihydroxycholecalciferol | ? | |
| additional information | the 25-hydroxy group is essential for 1alpha-hydroxylating activity, the 24-hydroxy group enhances the activity, the 23-hydroxy group strongly reduces activity | Mus musculus | ? | - |
? | |
| additional information | the 25-hydroxy group is essential for 1alpha-hydroxylating activity, the 24-hydroxy group enhances the activity, the 23-hydroxy group strongly reduces activity | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYB27B1 | - |
Mus musculus |
| CYB27B1 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Mus musculus | |
| NADPH | - |
Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | 0.002 | vitamin D3 | - |
Mus musculus | |
| 0.001 | 0.002 | vitamin D3 | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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