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Literature summary for 1.14.15.3 extracted from

  • Loughran, P.A.; Roman, L.J.; Aitken, A.E.; Miller, R.T.; Masters, B.S.
    Identification of unique amino acids that modulate CYP4A7 activity (2000), Biochemistry, 39, 15110-15120.
    View publication on PubMed
Search for more literature for 1.14.15.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain XL1 and SCS1 Oryctolagus cuniculus

Protein Variants

Protein Variants Comment Organism
H206Y gene CYP4A7 Oryctolagus cuniculus
H206Y/S255F gene CYP4A7 Oryctolagus cuniculus
R90W gene CYP4A7 Oryctolagus cuniculus
R90W/W93S gene CYP4A7 Oryctolagus cuniculus
S255F gene CYP4A7 Oryctolagus cuniculus
W93S gene CYP4A7 Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe
-
 Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arachidonic acid + NADPH + H+ + O2 Oryctolagus cuniculus
-
 20-hydroxyeicosatetraenoic acid + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
 rabbit
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonic acid + NAD(P)H + H+ + O2
-
 Oryctolagus cuniculus 20-hydroxyicosa-5,8,11,14-tetraenoic acid + NAD(P)+ + H2O
-
 ?
arachidonic acid + NADPH + H+ + O2
-
 Oryctolagus cuniculus 20-hydroxyeicosatetraenoic acid + NADP+ + H2O
-
 ?
fatty acid + NAD(P)H + H+ + O2 laurate and arachidonic acid Oryctolagus cuniculus omega-hydroxy fatty acid + NAD(P)+ + H2O
-
 ?
fatty acid + NAD(P)H + H+ + O2 NADPH-P450 reductase and cytochrome b5 are part of the enzyme system Oryctolagus cuniculus omega-hydroxy fatty acid + NAD(P)+ + H2O
-
 ?
lauric acid + NAD(P)H + H+ + O2
-
 Oryctolagus cuniculus 12-hydroxydodecanoic acid + NAD(P)+ + H2O
-
 ?
prostaglandin E1 + NADPH + O2
-
 Oryctolagus cuniculus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0267
-
 laurate CYP4A7, R90W mutant, omega-hydroxylation Oryctolagus cuniculus
0.117
-
 laurate CYP4A4wt, omega-hydroxylation Oryctolagus cuniculus
0.167
-
 arachidonic acid CYP4A7, R90W mutant, omega-hydroxylation Oryctolagus cuniculus
0.183
-
 prostaglandin E1 CYP4A7wt, omega-hydroxylation Oryctolagus cuniculus
0.2
-
 laurate CYP4A7, R90W/W93S mutant, omega-hydroxylation Oryctolagus cuniculus
0.217
-
 arachidonic acid CYP4A7, R90W/W93S mutant, omega-hydroxylation Oryctolagus cuniculus
0.25
-
 prostaglandin E1 CYP4A7, R90W/W93S mutant, omega-hydroxylation Oryctolagus cuniculus
0.283
-
 prostaglandin E1 CYP4A7, R90W mutant, omega-hydroxylation Oryctolagus cuniculus
0.3
-
 prostaglandin E1 CYP4A7, W93S mutant, omega-hydroxylation Oryctolagus cuniculus
0.3
-
 prostaglandin E1 CYP4A7, S255F mutant, omega-hydroxylation Oryctolagus cuniculus
0.3
-
 prostaglandin E1 CYP4A7, H206Y/S255F mutant, omega-hydroxylation Oryctolagus cuniculus
0.383
-
 prostaglandin E1 CYP4A4wt, omega-hydroxylation Oryctolagus cuniculus
0.417
-
 prostaglandin E1 CYP4A7, H206Y mutant, omega-hydroxylation Oryctolagus cuniculus
0.45
-
 arachidonic acid CYP4A7, W93S mutant, omega-hydroxylation Oryctolagus cuniculus
0.5
-
 laurate CYP4A7, W93S mutant, omega-hydroxylation Oryctolagus cuniculus
0.717
-
 arachidonic acid CYP4A7wt, omega-hydroxylation Oryctolagus cuniculus
0.767
-
 laurate CYP4A7wt, omega-hydroxylation Oryctolagus cuniculus
0.783
-
 arachidonic acid CYP4A4wt, omega-hydroxylation Oryctolagus cuniculus
2.15
-
 arachidonic acid CYP4A7, S255F mutant, omega-hydroxylation Oryctolagus cuniculus
2.17
-
 arachidonic acid CYP4A7, H206Y/S255F mutant, omega-hydroxylation Oryctolagus cuniculus
2.23
-
 laurate CYP4A7, H206Y mutant, omega-hydroxylation Oryctolagus cuniculus
2.33
-
 arachidonic acid CYP4A7, H206Y mutant, omega-hydroxylation Oryctolagus cuniculus
2.45
-
 laurate CYP4A7, H206Y/S255F mutant, omega-hydroxylation Oryctolagus cuniculus
2.67
-
 laurate CYP4A7, S255F mutant, omega-hydroxylation Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Oryctolagus cuniculus