Literature summary for 1.14.15.3 extracted from

Hoefer, R.; Briesen, I.; Beck, M.; Pinot, F.; Schreiber, L.; Franke, R.
The Arabidopsis cytochrome P450 CYP86A1 encodes a fatty acid omega-hydroxylase involved in suberin monomer biosynthesis (2008), J. Exp. Bot., 59, 2347-2360.

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Cloned(Commentary)

Cloned (Comment)	Organism
the 1.4 kb putative promoter region upstream of the predicted CYP86A1 start codon fused in frame to the beta-glucuronidase gene and used to transform wild-type Arabidopsis under the control of the native promoter. Transgenic expression of CYP86A1 fused to GFP, CYP86A1:GFP heterologously and transiently expressed under the control of the CaMV35S-promoter in the epidermis of Nicotiana benthamiana leaves	Arabidopsis thaliana

Cloned (Comment)

Organism

the 1.4 kb putative promoter region upstream of the predicted CYP86A1 start codon fused in frame to the beta-glucuronidase gene and used to transform wild-type Arabidopsis under the control of the native promoter. Transgenic expression of CYP86A1 fused to GFP, CYP86A1:GFP heterologously and transiently expressed under the control of the CaMV35S-promoter in the epidermis of Nicotiana benthamiana leaves

Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	transgenic expression of CYP86A1 fused to GFP distributes CYP86A1 to the endoplasmic reticulum, indicating that suberin monomer biosynthesis takes place in this sub-cellular compartment before intermediates are exported in the apoplast	Arabidopsis thaliana	5783	-

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	P48422	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	no detectable CYP86A1 transcript levels in above-ground organs such as leaves, stems, flowers, and siliques	Arabidopsis thaliana	-
root	strong root specificity and a localized expression in the root endodermis, high expression levels in all three root zones from the tip to the base	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
suberin + NADPH + H+ + O2	-	Arabidopsis thaliana	? + NADP+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
CYP86A1	-	Arabidopsis thaliana
Fatty acid omega-hydroxylase	-	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Arabidopsis thaliana

General Information

General Information	Comment	Organism
physiological function	is a key enzyme for aliphatic root suberin biosynthesis. In contrast to wild-type, no CYP86A1 transcript in total RNA from horst-1 seedlings (carries the T-DNA insertion in the first exon of CYP86A1) and horst-2 seedlings (T-DNA insertion is located in the second exon). Complemented CYP86A1-transformed horst-1 plants reveal a quantitative and qualitative aliphatic suberin composition similar to the wild-type	Arabidopsis thaliana