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Literature summary for 1.14.15.4 extracted from

  • Watanuki, M.; Tilley, B.E.; Hall, P.F.
    Purification and properties of cytochrome p-450 (11beta- and 18-hydroxylase) from bovine adrenocortical mitochondria (1977), Biochim. Biophys. Acta, 483, 236-247.
    View publication on PubMed

General Stability

General Stability Organism
deoxycorticosterone stabilizes Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
CO
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
deoxycorticosterone identical for both enzymes activities Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Bos taurus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47500
-
4 * 47500, SDS-PAGE Bos taurus
47500
-
x * 47500, SDS-PAGE Bos taurus
185000
-
exclusion chromatography Bos taurus
1000000
-
glycerol density gradient Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bos taurus corticosterone generally believed to be preferred substrate for 18-hydroxylase ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
copurification of 11beta-hydroxylase with 18-hydroxylase, ammonium sulfate, DEAE-chromatography Bos taurus

Reaction

Reaction Comment Organism Reaction ID
a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O both hydroxylation activities seem to occur in a single enzyme Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal cortex
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.001061
-
deoxycorticosterone, 18-hydroxylation activity in Bos taurus
0.01193
-
deoxycorticosterone, 11beta-hydroxylation activity Bos taurus

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, 1 week, without significant loss of activity Bos taurus
4°C, 1 week, without significant loss of activity Bos taurus
4°C, sonicated mitochondria, stable Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-deoxycorticosterone + reduced adrenal ferredoxin + O2 addition of adrenodoxin and adrenodoxin reductase Bos taurus corticosterone + oxidized adrenal ferredoxin + H2O
-
?
corticosterone + reduced adrenal ferredoxin + O2 addition of adrenodoxin and adrenodoxin reductase Bos taurus 18-hydroxycorticosterone + oxidized adrenal ferredoxin + H2O
-
?
additional information corticosterone generally believed to be preferred substrate for 18-hydroxylase Bos taurus ?
-
?

Subunits

Subunits Comment Organism
? x * 47500, SDS-PAGE Bos taurus
tetramer 4 * 47500, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Bos taurus