General Stability | Organism |
---|---|
deoxycorticosterone stabilizes | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | 10%, v/v, complete inhibition of hydroxylation | Bos taurus | |
antiserum | produced by rabbits | Bos taurus | |
CO | - |
Bos taurus | |
deoxycorticosterone | competitive substrate inhibition | Bos taurus | |
ethanol | 10%, v/v, complete inhibition of hydroxylation | Bos taurus | |
KCl | total inactivation | Bos taurus | |
methanol | 10%, v/v, complete inhibition of hydroxylation | Bos taurus | |
Metyrapone | competitive substrate inhibition | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bos taurus | corticosterone normal substrate in vivo | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O | both hydroxylation activities seem to occur in a single enzyme | Bos taurus | |
a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O | heme protein of so-called P-450 family | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000171 | - |
18-hydroxylation activity, 4% O2, 96% N2 | Bos taurus |
0.000211 | - |
18-hydroxylation activity, 21% O2, 79% N2 | Bos taurus |
0.00205 | - |
11beta-hydroxylation activity, 4% O2, 96% N2 | Bos taurus |
0.00278 | - |
11beta-hydroxylation activity, 21% O2, 79% N2 | Bos taurus |
Storage Stability | Organism |
---|---|
4°C, 11 days, both activities decrease considerably in a similar way, deoxycorticosterone delays decline | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + reduced adrenal ferredoxin + O2 | addition of adrenodoxin and adrenodoxin reductase | Bos taurus | corticosterone + oxidized adrenal ferredoxin + H2O | - |
? | |
corticosterone + reduced adrenal ferredoxin + O2 | addition of adrenodoxin and adrenodoxin reductase | Bos taurus | 18-hydroxy-11-deoxycorticosterone + oxidized adrenal ferredoxin + ? | - |
? | |
additional information | corticosterone normal substrate in vivo | Bos taurus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Bos taurus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
2 | 50 | at 50°C almost no activity | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
2 | - |
14% residual activity of 11beta- and 18-hydroxylation | Bos taurus |
30 | - |
after preincubation for 20 min, 27% residual activity, after 60 min 5.7% | Bos taurus |
50 | - |
complete inactivation | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Bos taurus |