Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49500 | - |
x * 49500 of capsular portion and x * 51500 of capsular and decapsular portion, 11beta-hydroxylase activities, SDS-PAGE | Rattus norvegicus |
51500 | - |
x * 49500 of capsular portion and x * 51500 of capsular and decapsular portion, 11beta-hydroxylase activities, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male, Sprague-Dawley, low sodium, high potassium treated | - |
Purification (Comment) | Organism |
---|---|
cholate extraction, hydroxyapatite HPLC | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | capsular portion in zona glomerulosa cells, decapsular in zonae fasciculata and reticularis | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
several activities of 49.5 and 51.5 kDa proteins for multihydroxylation steps | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 | 49 kDa protein, exclusively in zona glomerulosa, 11beta -hydroxylation, as well as 18-hydroxylation and 18-hydroxydehydrogenation of corticosterone with 18-hydroxydeoxycorticosterone and 18-hydroxycorticosterone as intermediates | Rattus norvegicus | aldosterone + oxidized adrenal ferredoxin + 2 H2O | - |
? | |
11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 | by 49.5 kDa protein from capsular portion, 11beta-/18-hydroxylation by 51.5 kDa protein | Rattus norvegicus | aldosterone + oxidized adrenal ferredoxin + 2 H2O | - |
? | |
11-deoxycorticosterone + reduced adrenal ferredoxin + O2 | by 49.5 kDa protein from capsular portion, 11beta-/18-hydroxylation by 51.5 kDa protein | Rattus norvegicus | corticosterone + oxidized adrenal ferredoxin + H2O | - |
? | |
11-deoxycorticosterone + reduced adrenal ferredoxin + O2 | by 49.5 kDa protein from capsular portion, 11beta-/18-hydroxylation by 51.5 kDa protein | Rattus norvegicus | corticosterone + oxidized adrenal ferredoxin + H2O | 51.5 kDa protein | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49500 of capsular portion and x * 51500 of capsular and decapsular portion, 11beta-hydroxylase activities, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Rattus norvegicus |