Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Anti-11beta-hydroxylase IgG | polyclonal, raised in rabbits, inhibition of 11-beta/18-hydroxylation and aldosterone synthesis of corticosterone | Bos taurus | |
Metyrapone | - |
Bos taurus | |
Metyrapone | inhibition of the purified 11beta-hydroxylase, 18-hydroxylation and aldosterone synthesis of corticosterone are inhibited | Sus scrofa | |
SKF 525A | little inhibition of the purified 11beta-hydroxylase, 18-hydroxylation and aldosterone synthesis of corticosterone are inhibited | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | from zona fasciculata | Bos taurus | 5739 | - |
mitochondrion | from zona glomerulosa | Bos taurus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, two-dimensional gel | Sus scrofa |
47500 | - |
x * 47500 | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
copurification of 11beta-/18-hydroxylase, cholate extraction, ammonium sulfate precipitation, Tween extraction, octymine-Sepharose | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Sus scrofa | - |
adrenal cortex | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00003 | - |
18-hydroxylation | Bos taurus |
0.0053 | - |
11beta-hydroxylation | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 | - |
Bos taurus | aldosterone + oxidized adrenal ferredoxin + 2 H2O | - |
? | |
11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 | supported by adrenodoxin and adrenodoxin reductase, 3 step-hydroxylation with corticosterone and 18-hydroxycorticosterone as intermediates, 11beta-/18-hydroxylase | Sus scrofa | aldosterone + oxidized adrenal ferredoxin + 2 H2O | - |
? | |
corticosterone + reduced adrenal ferredoxin + O2 | 18-hydroxycorticosterone as intermediate of 11beta-hydroxylation | Bos taurus | aldosterone + oxidized adrenal ferredoxin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, two-dimensional gel | Sus scrofa |
? | x * 47500 | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sus scrofa |
30 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Sus scrofa | |
NADPH | - |
Bos taurus |