Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | inner membrane, all 3 zones | Bos taurus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Na-cholate extraction, ammonium sulfate precipitation, aniline-Sepharose chromatography, hydroxylapatite HPLC | Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O | 11beta-/18-hydroxylation and aldosterone synthesis are catalyzed by 2 distinct forms of cytochrome P-45011beta | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 | 11beta-hydroxylase and 18-hydroxylase activities, after replacement of Tween 20 by phosphatidylcholine catalytic activity for aldosterone activity is exhibited, form 1 higher active in aldosterone and 18-hydroxycorticosterone production and less active in the production of corticosterone and 18-hydroxydeoxycorticosterone from deoxycorticosterone as form 2 | Bos taurus | aldosterone + oxidized adrenal ferredoxin + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48500-51500, SDS-PAGE after Sepharose, x * 48400/49500, SDS-PAGE after HPLC | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
cytochrome P-45011-beta | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | adrenodoxin and NADPH-linked adrenodoxin reductase | Bos taurus |