Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Anti-11beta-hydroxylase IgG | cross-reaction with 51 kDa protein | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
x * 49000 and x * 51000, SDS-PAGE | Rattus norvegicus |
51000 | - |
x * 49000 and x * 51000, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male, Zur:SIV low sodium, high potassium or high sodium, low potassium treated for stimulation or supprssion, respectively | - |
Purification (Comment) | Organism |
---|---|
octyl-Sepharose chromatography, cholate extraction | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O | 11beta-/18-hydroxylation and aldosterone synthesis are catalyzed by 2 distinct forms of cytochrome P-45011beta | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | capsular portion in zona glomerulosa cells, decapsular in zonae fasciculata and reticularis | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
conversion of 11-deoxycorticosterone | Rattus norvegicus |
0.00000314 | - |
aldosterone production, mitochondria from zona glomerulosa | Rattus norvegicus |
0.0000236 | - |
18-hydroxycorticosterone production, mitochondria from zona fasciculata | Rattus norvegicus |
0.000119 | - |
18-hydroxycorticosterone production, mitochondria from zona glomerulosa | Rattus norvegicus |
0.00206 | - |
18-hydroxydeoxycorticosterone production, mitochondria from zona glomerulosa | Rattus norvegicus |
0.00599 | - |
18-hydroxydeoxycorticosterone production, mitochondria from zona fasciculata | Rattus norvegicus |
0.00691 | - |
corticosterone production, mitochondria from zona glomerulosa | Rattus norvegicus |
0.00951 | - |
corticosterone production, mitochondria from zona fasciculata | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 | 49 kDa protein, exclusively in zona glomerulosa, 11beta -hydroxylation, as well as 18-hydroxylation and 18-hydroxydehydrogenation of corticosterone with 18-hydroxydeoxycorticosterone and 18-hydroxycorticosterone as intermediates | Rattus norvegicus | aldosterone + oxidized adrenal ferredoxin + 2 H2O | product of 49 kDa protein | ? | |
11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 | 51 kDa protein, zona fasciculata and glomerulosa, high sodium and low potassium, 11beta-/18-hydroxylation | Rattus norvegicus | aldosterone + oxidized adrenal ferredoxin + 2 H2O | product of 49 kDa protein | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000 and x * 51000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Rattus norvegicus |