Activating Compound | Comment | Organism | Structure |
---|---|---|---|
malate | plus NADP+ and in synergism with ascorbate plus NADH increase of aldosterone synthetase activity in zona glomerulosa, no stimulation of 11beta-/18-hydroxylation | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbate | without NADH, inhibition of aldosterone synthetase | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | from zona fasciculata | Bos taurus | 5739 | - |
mitochondrion | from zona glomerulosa and fasciculata | Bos taurus | 5739 | - |
mitochondrion | from zona glomerulosa | Bos taurus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O | for synthesis of aldosterone 3 molecules P450 must be reduced or one molecule must be reduced 3 times | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 | 3 steps in glomerulosa catalysed by one enzyme, 11beta-hydroxylation to corticosterone, 18-hydroxylation to 18-hydroxycorticosterone and aldehyde synthesis to aldosterone | Bos taurus | aldosterone + oxidized adrenal ferredoxin + 2 H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | plus NADH very low conversion and no aldosterone production, in synergism with NADH, malate and NADP+ increase of aldosterone synthetase activity in zona glomerulosa, not zona fasciculate | Bos taurus | |
NADH | - |
Bos taurus | |
NADP+ | plus malate supports all 4 conversions, increase of aldosterone synthetase activity | Bos taurus | |
NADPH | - |
Bos taurus |