Activating Compound | Comment | Organism | Structure |
---|---|---|---|
lipid extract | from adrenocortical mitochondria, increase of aldosterone production from corticosterone | Bos taurus |
Application | Comment | Organism |
---|---|---|
medicine | inhibitors for P-45011beta, against aldosterone overproduction which leads to oedematous diseases and hypertension | Bos taurus |
Cloned (Comment) | Organism |
---|---|
2 P-45011beta cDNAs, pc P-450 11beta-3 and -2, cloned into and expressed in COS-7 cells | Bos taurus |
General Stability | Organism |
---|---|
phospholipid vesicles, 0.2% soybean lecithin, added to purified P-45011beta for storage and during incubation | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
18-ethynylprogesterone | mechanism-based inhibition; weaker than 18-vinylprogesterone, inhibitor of aldosterone synthesis for both activities, stronger for 18-hydroxylation inhibition | Bos taurus | |
18-vinylprogesterone | competitive, potent inhibitor of aldosterone synthesis for both activities, 18-hydroxylation more effected, 65% inhibition of corticosterone and 11-deoxy-18-hydroxycorticosterone production by 0.03 mM; mechanism-based inhibition | Bos taurus | |
corticosterone | competitive inhibition of 11beta-hydroxylase activity | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters only slightly modified by presence of phospholipids, 0.2% soybean lecithin | Bos taurus | |
0.00087 | - |
11-deoxycorticosterone | without phospholipids | Bos taurus | |
0.0011 | - |
11-deoxycorticosterone | with phospholipids | Bos taurus | |
0.0015 | - |
11-deoxycorticosterone | +/-0.00008 | Bos taurus | |
0.0084 | - |
corticosterone | without phospholipids | Bos taurus | |
0.009 | - |
corticosterone | +/-0.0009 | Bos taurus | |
0.087 | - |
corticosterone | with phospholipids | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48500 | - |
x * 48500 major form, x * 49500 isoform, SDS-PAGE | Bos taurus |
49500 | - |
x * 48500 major form, x * 49500 isoform, SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
copurification of P-450 11beta-2 and 11beta-3, ammonium sulfate precipitation, octyl-Sepharose, separation of 2 isoforms by hydroxyapatite column connected to an FPLC system | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
-80°C, after ammonium sulfate precipitation, 50 mM potassium phosphate, pH 7.4, 0.1 mM EDTA, 0.1 mM dithiothreitol, 0.5% sodium cholate, 0.5% soybean lecithin, several months, without loss of activity | Bos taurus |
-80°C, after hydroxyapatite column/FPLC system, 50 mM potassium phosphate, pH 7.4, 0.01% sodium cholate, 0.05% soybean lecithin, 0.01 mM 11-deoxycorticosterone, 0.1 mM dithiothreitol, 0.1 mM EDTA, without loss of activity | Bos taurus |
-80°C, after octyl-Sepharose, deoxycorticosterone-bound, 50 mM potassium phosphate, pH 7.4, 0.5% sodium cholate, 0.2% Tween 20, 0.01 mM 11-deoxycorticosterone, 0.1 mM dithiothreitol, 0.1 mM EDTA | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + reduced adrenal ferredoxin + O2 | 11beta-hydroxylation | Bos taurus | corticosterone + oxidized adrenal ferredoxin + H2O | - |
? | |
18-hydroxycorticosterone + reduced adrenal ferredoxin + O2 | - |
Bos taurus | aldosterone + oxidized adrenal ferredoxin + H2O | 18-hydroxylation | ? | |
corticosterone + reduced adrenal ferredoxin + O2 | 18-hydroxylation | Bos taurus | 18-hydroxycorticosterone + oxidized adrenal ferredoxin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48500 major form, x * 49500 isoform, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
in presence of phospholipids, catalytic activity stable to extended incubation and increased resistance of hemoprotein to denaturation | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0378 | - |
corticosterone | +/-0.2268, calculatetd from Kcat/Km | Bos taurus | |
0.66 | - |
11-deoxycorticosterone | +/-2.112, calculatetd from Kcat/Km | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | adrenodoxin and NADPH-linked adrenodoxin reductase | Bos taurus |