Application | Comment | Organism |
---|---|---|
medicine | inhibitors for P-45011beta, against aldosterone overproduction which leads to oedematous diseases and hypertension | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
18-ethynylprogesterone | suicide-substrate of aldosterone biosythesis, inhibits more strongly the 18-hydroxylation step | Bos taurus | |
18-vinyldeoxycorticosterone | deoxycorticosterone analog, very strong and reversible inhibitor for deoxycorticosterone and corticosterone oxidation, 0.001 mM leads to decrease in corticosterone production with 30% of total activity after 1 min, 100fold more efficient than 18-vinylprogesterone for inhibition of 11beta-hydroxylation step, only 6fold of more inhibition of 18-hydroxylation step | Bos taurus | |
18-vinylprogesterone | with 0.001 mM no inhibition detectable, with 0.01 mM 23% decrease in corticosterone production, better suicide-substrate of aldosterone biosythesis than 18-ethynylprogesterone, inhibits more strongly the 18-hydroxylation step | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00197 | - |
11-deoxycorticosterone | +/-0.00026 | Bos taurus | |
0.0059 | - |
corticosterone | +/-0.00018 | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
copurification of P-450 11beta-2 and 11beta-3, ammonium sulfate precipitation, octyl-Sepharose, separation of 2 isoforms by hydroxyapatite column connected to an FPLC system | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + reduced adrenal ferredoxin + O2 | 11beta-hydroxylation | Bos taurus | corticosterone + oxidized adrenal ferredoxin + H2O | - |
? | |
corticosterone + reduced adrenal ferredoxin + O2 | 18-hydroxylation | Bos taurus | aldosterone + oxidized adrenal ferredoxin + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | adrenodoxin and NADPH-linked adrenodoxin reductase | Bos taurus |