Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | progesterone induces the enzyme | Absidia caerulea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Absidia caerulea | |
Mg2+ | - |
Absidia caerulea | |
Mn2+ | - |
Absidia caerulea | |
Zn2+ | - |
Absidia caerulea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | highly activating | Absidia caerulea | |
Fe2+ | highly activating | Absidia caerulea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Absidia caerulea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
16,17alpha-epoxyprogesterone + reduced adrenal ferredoxin + O2 | Absidia caerulea | - |
11beta-hydroxy-16,17alpha-epoxyprogesterone + adrenal ferredoxin + H2O | the product is an important intermediate for many anti-inflammatory drugs | ? | |
16,17alpha-epoxyprogesterone + reduced adrenal ferredoxin + O2 | Absidia caerulea IBL02 | - |
11beta-hydroxy-16,17alpha-epoxyprogesterone + adrenal ferredoxin + H2O | the product is an important intermediate for many anti-inflammatory drugs | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Absidia caerulea | - |
- |
- |
Absidia caerulea IBL02 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by anion exchange and hydrophobic interaction chromatography, and two additonal steps of anion exchange chromatography | Absidia caerulea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Absidia caerulea | - |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, 24 h, addition of 20% glycerol, 0.5 mM PMSF, 0.5 mM DTT, 95.3% remaining activity | Absidia caerulea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
16,17alpha-epoxyprogesterone + reduced adrenal ferredoxin + O2 | - |
Absidia caerulea | 11beta-hydroxy-16,17alpha-epoxyprogesterone + adrenal ferredoxin + H2O | the product is an important intermediate for many anti-inflammatory drugs | ? | |
16,17alpha-epoxyprogesterone + reduced adrenal ferredoxin + O2 | assay optimization | Absidia caerulea | 11beta-hydroxy-16,17alpha-epoxyprogesterone + adrenal ferredoxin + H2O | - |
? | |
16,17alpha-epoxyprogesterone + reduced adrenal ferredoxin + O2 | - |
Absidia caerulea IBL02 | 11beta-hydroxy-16,17alpha-epoxyprogesterone + adrenal ferredoxin + H2O | the product is an important intermediate for many anti-inflammatory drugs | ? | |
16,17alpha-epoxyprogesterone + reduced adrenal ferredoxin + O2 | assay optimization | Absidia caerulea IBL02 | 11beta-hydroxy-16,17alpha-epoxyprogesterone + adrenal ferredoxin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Absidia caerulea |
Synonyms | Comment | Organism |
---|---|---|
11beta-hydroxylase | - |
Absidia caerulea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Absidia caerulea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
purified enzyme, loss of 75% activity after 3 h, and of all activity after 8 h | Absidia caerulea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Absidia caerulea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | cofactor screening, overview, the enzyme is active with NADPH | Absidia caerulea | |
reduced adrenal ferredoxin | - |
Absidia caerulea |