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Literature summary for 1.14.15.4 extracted from

  • Zoellner, A.; Kagawa, N.; Waterman, M.R.; Nonaka, Y.; Takio, K.; Shiro, Y.; Hannemann, F.; Bernhardt, R.
    Purification and functional characterization of human 11beta hydroxylase expressed in Escherichia coli (2008), FEBS J., 275, 799-810.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, coexpression with molecular chaperones GroES and GroEL Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Homo sapiens
0.002
-
adrenal ferredoxin pH 7.4, recombinant enzyme, with 11-deoxycorticosterone as substrate Homo sapiens
0.0024
-
adrenal ferredoxin pH 7.4, recombinant enzyme, with 11-deoxycortisol as substrate Homo sapiens
0.18
-
11-deoxycorticosterone pH 7.4, recombinant enzyme Homo sapiens
0.338
-
11-deoxycortisol pH 7.4, recombinant enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55761
-
x * 55761, recombinant enzyme, mass spectrometry Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
11-deoxycortisol + adrenal ferredoxin + H2O Homo sapiens
-
cortisol + reduced adrenal ferredoxin + O2
-
?
additional information Homo sapiens the enzyme is regulated by adrenocorticotrophic hormone, ACTH ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli to homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland zona fasiculata Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0198
-
purified recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-deoxycorticosterone + adrenal ferredoxin + H2O
-
Homo sapiens corticosterone + reduced adrenal ferredoxin + O2
-
?
11-deoxycortisol + adrenal ferredoxin + H2O
-
Homo sapiens cortisol + reduced adrenal ferredoxin + O2
-
?
additional information the enzyme is regulated by adrenocorticotrophic hormone, ACTH Homo sapiens ?
-
?
additional information the recombinant enzyme is capable of hydroxylating its substrates at position 11-beta Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 55761, recombinant enzyme, mass spectrometry Homo sapiens
More enzyme secondary structure, overview Homo sapiens

Synonyms

Synonyms Comment Organism
11beta hydroxylase
-
Homo sapiens
CYP11B1
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.167
-
11-deoxycortisol pH 7.4, recombinant enzyme Homo sapiens
0.85
-
11-deoxycorticosterone pH 7.4, recombinant enzyme Homo sapiens
1
-
adrenal ferredoxin pH 7.4, recombinant enzyme, with 11-deoxycorticosterone as substrate Homo sapiens
1.48
-
adrenal ferredoxin pH 7.4, recombinant enzyme, with 11-deoxycortisol as substrate Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
Ferredoxin [2Fe-2S] ferredoxin Homo sapiens