Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, coexpression with molecular chaperones GroES and GroEL | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Homo sapiens | |
0.002 | - |
adrenal ferredoxin | pH 7.4, recombinant enzyme, with 11-deoxycorticosterone as substrate | Homo sapiens | |
0.0024 | - |
adrenal ferredoxin | pH 7.4, recombinant enzyme, with 11-deoxycortisol as substrate | Homo sapiens | |
0.18 | - |
11-deoxycorticosterone | pH 7.4, recombinant enzyme | Homo sapiens | |
0.338 | - |
11-deoxycortisol | pH 7.4, recombinant enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55761 | - |
x * 55761, recombinant enzyme, mass spectrometry | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycortisol + adrenal ferredoxin + H2O | Homo sapiens | - |
cortisol + reduced adrenal ferredoxin + O2 | - |
? | |
additional information | Homo sapiens | the enzyme is regulated by adrenocorticotrophic hormone, ACTH | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli to homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | zona fasiculata | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0198 | - |
purified recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + adrenal ferredoxin + H2O | - |
Homo sapiens | corticosterone + reduced adrenal ferredoxin + O2 | - |
? | |
11-deoxycortisol + adrenal ferredoxin + H2O | - |
Homo sapiens | cortisol + reduced adrenal ferredoxin + O2 | - |
? | |
additional information | the enzyme is regulated by adrenocorticotrophic hormone, ACTH | Homo sapiens | ? | - |
? | |
additional information | the recombinant enzyme is capable of hydroxylating its substrates at position 11-beta | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55761, recombinant enzyme, mass spectrometry | Homo sapiens |
More | enzyme secondary structure, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
11beta hydroxylase | - |
Homo sapiens |
CYP11B1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.167 | - |
11-deoxycortisol | pH 7.4, recombinant enzyme | Homo sapiens | |
0.85 | - |
11-deoxycorticosterone | pH 7.4, recombinant enzyme | Homo sapiens | |
1 | - |
adrenal ferredoxin | pH 7.4, recombinant enzyme, with 11-deoxycorticosterone as substrate | Homo sapiens | |
1.48 | - |
adrenal ferredoxin | pH 7.4, recombinant enzyme, with 11-deoxycortisol as substrate | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | [2Fe-2S] ferredoxin | Homo sapiens |