Cloned (Comment) | Organism |
---|---|
expression of C-terminally His6-tagged CYP11B2 mature form without the mitochondrial targeting sequence of 24 residues at the N-terminus in Escherichia coli, co-expression with the molecular chaperones GroEL and GroES | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ketoconazole | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, recombinant His-tagged enzyme without the mitochondrial targeting sequence, SDS-PAGE, x * 55890, sequence calculation of enzyme without the mitochondrial targeting sequence but with His6-tag | Homo sapiens |
55890 | - |
x * 50000, recombinant His-tagged enzyme without the mitochondrial targeting sequence, SDS-PAGE, x * 55890, sequence calculation of enzyme without the mitochondrial targeting sequence but with His6-tag | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + reduced adrenodoxin + O2 | Homo sapiens | - |
corticosterone + oxidized adrenodoxin + H2O | - |
? | |
18-hydroxycorticosterone + reduced adrenodoxin + O2 | Homo sapiens | - |
aldosterone + oxidized adrenodoxin + H2O | - |
? | |
corticosterone + reduced adrenodoxin + O2 | Homo sapiens | - |
18-hydroxycorticosterone + oxidized adrenodoxin + H2O | - |
? | |
additional information | Homo sapiens | bifunctional enzyme catalyzing the biosynthesis of aldosterone by successive 11beta- and 18-hydroxylation followed by an 18-oxidation of 11-deoxycorticosterone | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P19099 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged CYP11B2 from Escherichia coli by nickel affinity chromatography and anion and cation exchange chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + reduced adrenodoxin + O2 | - |
Homo sapiens | corticosterone + oxidized adrenodoxin + H2O | - |
? | |
18-hydroxycorticosterone + reduced adrenodoxin + O2 | - |
Homo sapiens | aldosterone + oxidized adrenodoxin + H2O | - |
? | |
corticosterone + reduced adrenodoxin + O2 | - |
Homo sapiens | 18-hydroxycorticosterone + oxidized adrenodoxin + H2O | - |
? | |
additional information | bifunctional enzyme catalyzing the biosynthesis of aldosterone by successive 11beta- and 18-hydroxylation followed by an 18-oxidation of 11-deoxycorticosterone | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, recombinant His-tagged enzyme without the mitochondrial targeting sequence, SDS-PAGE, x * 55890, sequence calculation of enzyme without the mitochondrial targeting sequence but with His6-tag | Homo sapiens |
More | homology modeling and molecular docking of CYP11B2, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Aldosterone synthase | - |
Homo sapiens |
CYP11B2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | human aldosterone synthase is responsible for the biosynthesis of aldosterone from 11-deoxycorticosterone in a sequential three-step reaction with two intermediates, corticosterone and 18-hydroxycorticosterone | Homo sapiens |
additional information | homology modeling and molecular docking of CYP11B2, overview | Homo sapiens |