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Literature summary for 1.14.15.4 extracted from

  • Adams, B.P.; Bose, H.S.
    Alteration in accumulated aldosterone synthesis as a result of N-terminal cleavage of aldosterone synthase (2012), Mol. Pharmacol., 81, 465-474.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of the different enzyme-DHFR fusion constructs in NCI-H295 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information generation of different enzyme-DHFR fusion constructs, where the full-length DHFR is fused to the N-terminally varying length of enzyme, analysis of subcellular localization after recombinant expression, overview. The rate of enzyme translocation is independent of the targeting sequence Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane
-
Homo sapiens 5743
-
mitochondrial intermembrane space
-
Homo sapiens 5758
-
mitochondrion the precursor form of the enzyme translocates into the matrix of the mitochondria, where it undergoes cleavage by mitochondrial processing peptidase to a mature form of approximately 54 kDa. Mature enzyme seems to translocate across the inner mitochondrial membrane a second time to finally reside in the intermembrane space Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens N-terminally unprocessed enzyme has 2fold more activity than the mature physiological enzyme ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adrenal cortex steroidogenic cell isolated mitochondria from human NCI-H295 adrenal cells Homo sapiens
-
MA-10 cell
-
Homo sapiens
-
NCI-H295 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information N-terminally unprocessed enzyme has 2fold more activity than the mature physiological enzyme Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
Aldosterone synthase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

Expression

Organism Comment Expression
Homo sapiens stimulation of enzyme expression by the renin-angiotensin system, which takes 12 h to reach full effect, and then 24 h to subside up

General Information

General Information Comment Organism
malfunction overproduction of aldosterone in the adrenal gland can lead to hypertension, a major cause of heart disease and stroke Homo sapiens
additional information N-terminal 26 amino acids of the enzyme target cargo protein DHFR to the mitochondria Homo sapiens
physiological function aldosterone synthase regulates blood volume by synthesizing the mineralocorticoid aldosterone. Subcellular mechanisms of enzyme localization relate to production of aldosterone and reveal a rapid, promoter-independent regulation of aldosterone production Homo sapiens