Cloned (Comment) | Organism |
---|---|
expression of the different enzyme-DHFR fusion constructs in NCI-H295 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of different enzyme-DHFR fusion constructs, where the full-length DHFR is fused to the N-terminally varying length of enzyme, analysis of subcellular localization after recombinant expression, overview. The rate of enzyme translocation is independent of the targeting sequence | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Homo sapiens | 5743 | - |
mitochondrial intermembrane space | - |
Homo sapiens | 5758 | - |
mitochondrion | the precursor form of the enzyme translocates into the matrix of the mitochondria, where it undergoes cleavage by mitochondrial processing peptidase to a mature form of approximately 54 kDa. Mature enzyme seems to translocate across the inner mitochondrial membrane a second time to finally reside in the intermembrane space | Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | N-terminally unprocessed enzyme has 2fold more activity than the mature physiological enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex steroidogenic cell | isolated mitochondria from human NCI-H295 adrenal cells | Homo sapiens | - |
MA-10 cell | - |
Homo sapiens | - |
NCI-H295 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | N-terminally unprocessed enzyme has 2fold more activity than the mature physiological enzyme | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Aldosterone synthase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | stimulation of enzyme expression by the renin-angiotensin system, which takes 12 h to reach full effect, and then 24 h to subside | up |
General Information | Comment | Organism |
---|---|---|
malfunction | overproduction of aldosterone in the adrenal gland can lead to hypertension, a major cause of heart disease and stroke | Homo sapiens |
additional information | N-terminal 26 amino acids of the enzyme target cargo protein DHFR to the mitochondria | Homo sapiens |
physiological function | aldosterone synthase regulates blood volume by synthesizing the mineralocorticoid aldosterone. Subcellular mechanisms of enzyme localization relate to production of aldosterone and reveal a rapid, promoter-independent regulation of aldosterone production | Homo sapiens |