Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cardiolipin | affinity of P-450scc for cholesterol is increased | Bos taurus | |
fatty acid | C18, natural detergents in DMPC vesicles, stimulation similar to octyl glucoside | Bos taurus | |
octyl glucoside | high concentrations of this detergent cause 50% stimulation of cholesterol side-chain-cleavage in large unilamellar vesicles at low cholesterol concentration, 0.01 mM increase the proportion of P-450 bound by cholesterol | Bos taurus | |
Phospholipid | P-450scc reconstituted in vesicles, cholate-solubilized for small unilamellar dioleoylphosphatidylcholine vesicles, non-ionic detergent n-octyl glucoside used for large ones | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
cholesterol | low concentration, both vesicle types | Bos taurus | |
0.3 | - |
cholesterol | large unilamellar vesicles, second type of reaction | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | matrix side | Bos taurus | 5743 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 6 reduced adrenodoxin + 3 O2 | Bos taurus | first step in biosynthesis of all steroid hormones | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, affinity chromatography | Bos taurus |
DEAE-cellulose, adrenodoxin-Sepharose | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 6 reduced adrenodoxin + 3 O2 | first step in biosynthesis of all steroid hormones | Bos taurus | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | - |
? | |
cholesterol + reduced adrenodoxin + O2 | - |
Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? | |
additional information | transfer of cholesterol to cytochrome P-450scc is rate-limiting | Bos taurus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | for small unilamellar vesicles 5fold lower | Bos taurus | |
0.417 | 0.5 | cholesterol | by large unilamellar vesicles | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | required | Bos taurus | |
NADPH | - |
Bos taurus |