Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Sus scrofa |
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bos taurus |
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Capra aegagrus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Sus scrofa | 5739 | - |
mitochondrion | - |
Bos taurus | 5739 | - |
mitochondrion | - |
Capra aegagrus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | cytochrome P450 enzyme | Sus scrofa | |
Fe2+ | cytochrome P450 enzyme | Bos taurus | |
Fe2+ | cytochrome P450 enzyme | Capra aegagrus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, SDS-PAGE | Sus scrofa |
52000 | - |
x * 52000, SDS-PAGE | Bos taurus |
52000 | - |
x * 52000, SDS-PAGE | Capra aegagrus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + reduced adrenal ferredoxin + O2 | Sus scrofa | - |
pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin | - |
? | |
cholesterol + reduced adrenal ferredoxin + O2 | Bos taurus | - |
pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin | - |
? | |
cholesterol + reduced adrenal ferredoxin + O2 | Capra aegagrus | - |
pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin | - |
? | |
additional information | Sus scrofa | the enzyme is important in steroidogenesis, regulatory mechanism, overview | ? | - |
? | |
additional information | Bos taurus | the enzyme is important in steroidogenesis, regulatory mechanism, overview | ? | - |
? | |
additional information | Capra aegagrus | the enzyme is important in steroidogenesis, regulatory mechanism, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Capra aegagrus | - |
hircus | - |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Sus scrofa |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Bos taurus |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Capra aegagrus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Sus scrofa | - |
adrenal gland | - |
Bos taurus | - |
corpus luteum | specific expressionin luteal cells, no expression in endothelial cells lining the blood vessels | Bos taurus | - |
luteal cell | - |
Bos taurus | - |
luteal gland | - |
Capra aegagrus | - |
additional information | immunohistochemic localization analysis, overview | Sus scrofa | - |
additional information | immunohistochemic localization analysis, overview | Bos taurus | - |
additional information | immunohistochemic localization analysis, overview | Capra aegagrus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + reduced adrenal ferredoxin + O2 | - |
Sus scrofa | pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin | - |
? | |
cholesterol + reduced adrenal ferredoxin + O2 | - |
Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin | - |
? | |
cholesterol + reduced adrenal ferredoxin + O2 | - |
Capra aegagrus | pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin | - |
? | |
additional information | the enzyme is important in steroidogenesis, regulatory mechanism, overview | Sus scrofa | ? | - |
? | |
additional information | the enzyme is important in steroidogenesis, regulatory mechanism, overview | Bos taurus | ? | - |
? | |
additional information | the enzyme is important in steroidogenesis, regulatory mechanism, overview | Capra aegagrus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, SDS-PAGE | Sus scrofa |
? | x * 52000, SDS-PAGE | Bos taurus |
? | x * 52000, SDS-PAGE | Capra aegagrus |
Synonyms | Comment | Organism |
---|---|---|
P450 cholesterol side-chain cleavage enzyme | - |
Sus scrofa |
P450 cholesterol side-chain cleavage enzyme | - |
Bos taurus |
P450 cholesterol side-chain cleavage enzyme | - |
Capra aegagrus |
P450scc | - |
Sus scrofa |
P450scc | - |
Bos taurus |
P450scc | - |
Capra aegagrus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Sus scrofa | |
cytochrome P450 | - |
Bos taurus | |
cytochrome P450 | - |
Capra aegagrus | |
reduced adrenal ferredoxin | - |
Sus scrofa | |
reduced adrenal ferredoxin | - |
Bos taurus | |
reduced adrenal ferredoxin | - |
Capra aegagrus |