Activating Compound | Comment | Organism | Structure |
---|---|---|---|
RNA | below 0.015 mg/ml activation, rat brain enzyme contains RNA, about 10% of the total mass | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
RNA | above 0.015 mg/ml | Bos taurus | |
RNA | above 0.015 mg/ml | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effect of RNA on Km | Rattus norvegicus | |
additional information | - |
additional information | effect of RNA on Km | Bos taurus | |
additional information | - |
additional information | rat brain: 2 kinetically distinguishable forms: low Km form, high Km form | Rattus norvegicus | |
0.0061 | - |
tyrosine | - |
Rattus norvegicus | |
0.08 | - |
Tetrahydropterin | low Km form | Rattus norvegicus | |
0.63 | - |
Tetrahydropterin | high Km form | Rattus norvegicus | |
0.95 | - |
6-Methyl-5,6,7,8-tetrahydropterin | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Bos taurus | |
phosphate | activated enzyme contains: 0.75 mol per mol of subunit | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61100 | - |
2 * 61100 + 2 * 62400, SDS-PAGE | Bos taurus |
62400 | - |
2 * 61100 + 2 * 62400, SDS-PAGE | Bos taurus |
63300 | - |
4 * 63300, SDS-PAGE | Rattus norvegicus |
210000 | 211000 | gel filtration | Bos taurus |
239000 | - |
brain, gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + tetrahydrobiopterin + O2 | Rattus norvegicus | rate-limiting step in catecholamine biosynthesis | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | Bos taurus | rate-limiting step in catecholamine biosynthesis | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2 kinetically distinguishable forms of the enzyme: low Km form and high Km form | Rattus norvegicus |
from adrenal medulla | Bos taurus |
from brain | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal medulla | - |
Rattus norvegicus | - |
adrenal medulla | - |
Bos taurus | - |
brain | - |
Rattus norvegicus | - |
brain | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0916 | - |
purified enzyme from brain | Rattus norvegicus |
0.18 | - |
purified enzyme from adrenal medulla | Bos taurus |
Storage Stability | Organism |
---|---|
-70°C, in presence of diisopropylfluorophosphate, 2 months | Bos taurus |
4°C, in presence of diisopropylfluorophosphate, 1 week | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + 6-methyltetrahydropterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
L-tyrosine + 6-methyltetrahydropterin + O2 | - |
Bos taurus | ? | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | 3,4-dihydroxy-L-phenylalanine is identical with dopa | ? | |
L-tyrosine + tetrahydrobiopterin + O2 | - |
Bos taurus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | 3,4-dihydroxy-L-phenylalanine is identical with dopa | ? | |
L-tyrosine + tetrahydrobiopterin + O2 | rate-limiting step in catecholamine biosynthesis | Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | rate-limiting step in catecholamine biosynthesis | Bos taurus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 63300, SDS-PAGE | Rattus norvegicus |
tetramer | 2 * 61100 + 2 * 62400, SDS-PAGE | Bos taurus |