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Literature summary for 1.14.16.2 extracted from

  • Ogawa, S.; Ichinose, H.
    Effect of metals and phenylalanine on the activity of human tryptophan hydroxylase-2: comparison with that on tyrosine hydroxylase activity (2006), Neurosci. Lett., 401, 261-265.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) as maltose-binding protein fusion protein Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 80% inhibition at 0.1 mM Homo sapiens
L-phenylalanine
-
Homo sapiens
Mn2+ 50% inhibition at 0.01 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
L-tyrosine 0-0.4 mM L-tyrosine, 1 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, 200 mM Na-HEPES pH 7.0, 100 mM 2-mercaptoethanol, 0.2 mg/ml catalase, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98900
-
recombinant enzyme, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
one chromatography step Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine + O2
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
hTH2
-
Homo sapiens
tyrosine hydroxylase
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.103
-
L-phenylalanine 0-0.4 mM L-tyrosine, 0-0.2 mM L-phenylalanine, 1 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, 200 mM Na-HEPES pH 7.0, 100 mM 2-mercaptoethanol, 0.2 mg/ml catalase, 37°C Homo sapiens