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Literature summary for 1.14.16.2 extracted from
- Identification and optimization of tyrosine hydroxylase activity in Mucuna pruriens DC. var. utilis (2010), Planta, 231, 1361-1369.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0041 | - |
tetrahydrobiopterin | pH 7.0, 37°C | Mucuna pruriens var. utilis |  |
| 0.8086 | - |
L-tyrosine | pH 6.0, 37°C | Mucuna pruriens var. utilis | |
| 1.1 | - |
tyramine | pH 7.0, 37°C | Mucuna pruriens var. utilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 2 mM | Mucuna pruriens var. utilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, SDS-PAGE, Western blot analysis | Mucuna pruriens var. utilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mucuna pruriens var. utilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| centrifugation | Mucuna pruriens var. utilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Mucuna pruriens var. utilis | - |
| pod | - |
Mucuna pruriens var. utilis | - |
| root | - |
Mucuna pruriens var. utilis | - |
| seed | immature cell | Mucuna pruriens var. utilis | - |
| stem | - |
Mucuna pruriens var. utilis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 101 | - |
L-tyramine, crude extract of mature leaves, pH 6.0, 37°C | Mucuna pruriens var. utilis |
| 113 | - |
L-tyrosine, crude extract of mature leaves, pH 6.0, 37°C | Mucuna pruriens var. utilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tyrosine + tetrahydrobiopterin + O2 | - |
Mucuna pruriens var. utilis | 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O | - |
? | |
| tyramine + tetrahydrobiopterin + O2 | - |
Mucuna pruriens var. utilis | dopamine + 4a-hydroxytetrahydrobiopterin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE, Western blot analysis | Mucuna pruriens var. utilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tyrosine 3-monooxygenase | - |
Mucuna pruriens var. utilis |
| tyrosine hydroxylase | - |
Mucuna pruriens var. utilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Mucuna pruriens var. utilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | 60 min | Mucuna pruriens var. utilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
broad optimum pH 4.0-7.5 | Mucuna pruriens var. utilis |
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