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Literature summary for 1.14.16.2 extracted from

  • Vermeer, L.M.; Higgins, C.A.; Roman, D.L.; Doorn, J.A.
    Real-time monitoring of tyrosine hydroxylase activity using a plate reader assay (2013), Anal. Biochem., 432, 11-15.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3,4-dihydroxyphenylacetaldehyde potent inhibitor, 0.01 and 0.02 mM cause 66% and 75% inhibition, respectively Homo sapiens
3-iodo-L-tyrosine competitive inhibitor Homo sapiens
CoCl2 0.1 mM CoCl2 results in more than 80% inhibition Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + tetrahydrobiopterin + O2 Homo sapiens
-
3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin column chromatography and HiPrep Q column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + tetrahydrobiopterin + O2
-
Homo sapiens 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?

Synonyms

Synonyms Comment Organism
tyrosine hydroxylase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
tetrahydrobiopterin
-
Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.05
-
pH and temperature not specified in the publication Homo sapiens 3-iodo-L-tyrosine