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Literature summary for 1.14.16.2 extracted from
- Catalytic domain surface residues mediating catecholamine inhibition in tyrosine hydroxylase (2014), J. Biochem., 155, 183-193.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A297L | the Vmax is significantly less reduced by dopamine than for the wild type enzyme | Homo sapiens |
| D361N | reductions in Vmax are not significantly different from the wild type enzyme | Homo sapiens |
| E362G | the Vmax is reduced compared to the wild type enzyme | Homo sapiens |
| E362Q | reductions in Vmax are not significantly different from the wild type enzyme | Homo sapiens |
| E362R/E365R | the Vmax is significantly less reduced by dopamine than for the wild type enzyme | Homo sapiens |
| E365G | the Vmax is reduced compared to the wild type enzyme | Homo sapiens |
| E365Q | the Vmax is significantly less reduced by dopamine than for the wild type enzyme | Homo sapiens |
| K170E/L480A | the mutant is inhibited over the same range of dopamine like the wild type enzyme | Homo sapiens |
| K366L | reductions in Vmax are not significantly different from the wild type enzyme | Homo sapiens |
| R37E/R38E | the KM for tetrahydrobiopterin measured for the mutant is approximately half that of the wild type enzyme and the Vmax is significantly less reduced by dopamine than for the wild type enzyme | Homo sapiens |
| S368A | the Vmax is significantly less reduced by dopamine than for the wild type enzyme | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dopamine | - |
Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0081 | - |
L-tyrosine | mutant enzyme R37E/R38E, pH and temperature not specified in the publication | Homo sapiens | |
| 0.014 | - |
L-tyrosine | mutant enzyme K366L, pH and temperature not specified in the publication | Homo sapiens | |
| 0.015 | - |
L-tyrosine | mutant enzyme E362R/E365R, pH and temperature not specified in the publication | Homo sapiens | |
| 0.016 | - |
L-tyrosine | wild type enzyme, pH and temperature not specified in the publication | Homo sapiens | |
| 0.016 | - |
L-tyrosine | mutant enzyme D361N, pH and temperature not specified in the publication | Homo sapiens | |
| 0.016 | - |
L-tyrosine | mutant enzyme E362G, pH and temperature not specified in the publication | Homo sapiens | |
| 0.019 | - |
L-tyrosine | mutant enzyme E365G, pH and temperature not specified in the publication | Homo sapiens | |
| 0.02 | - |
L-tyrosine | mutant enzyme A297L, pH and temperature not specified in the publication | Homo sapiens | |
| 0.025 | - |
L-tyrosine | mutant enzyme S368A, pH and temperature not specified in the publication | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
4 * 70000, SDS-PAGE | Homo sapiens |
| 300000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tyrosine + tetrahydrobiopterin + O2 | Homo sapiens | - |
3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P07101 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tyrosine + tetrahydrobiopterin + O2 | - |
Homo sapiens | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 70000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tyrosine hydroxylase | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| tetrahydrobiopterin | - |
Homo sapiens | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.000595 | - |
wild type enzyme, pH and temperature not specified in the publication | Homo sapiens | dopamine | |
| 0.000645 | - |
mutant enzyme K170E/L480A, pH and temperature not specified in the publication | Homo sapiens | dopamine | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme performs the rate-limiting step in catecholamine synthesis | Homo sapiens |
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Release 2023.1 (January 2023)
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