Literature summary for 1.14.16.2 extracted from

Higgins, C.A.; Vermeer, L.M.; Doorn, J.A.; Roman, D.L.
Expression and purification of recombinant human tyrosine hydroxylase as a fusion protein in Escherichia coli (2012), Protein Expr. Purif., 84, 219-223.

Search for more literature for 1.14.16.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
3,4-dihydroxyphenylacetaldehyde	potent inhibitor	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
56000	-	x * 56000, SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-tyrosine + tetrahydrobiopterin + O2	Homo sapiens	-	3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
amylose resin column chromatography and HiPrep Q column chromatography	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0054	-	crude enzyme, at pH 6.8 and 37°C	Homo sapiens
0.0163	-	purified enzyme, at pH 6.8 and 37°C	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-tyrosine + tetrahydrobiopterin + O2	-	Homo sapiens	3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 56000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
tyrosine hydroxylase	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
tetrahydrobiopterin	-	Homo sapiens

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Release 2023.1 (January 2023)