Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.16.4 extracted from

  • Fujisawa, H.; Nakata, H.
    Tryptophan 5-monooxygenase from rat brain stem (1987), Methods Enzymol., 142, 83-87.
    View publication on PubMed

General Stability

General Stability Organism
EDTA: 0.05 mM stabilizes Rattus norvegicus
glycerol, 10% stabilizes Rattus norvegicus
L-tryptophan stabilizes Rattus norvegicus
Tween 20: 0.06% stabilizes Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.119
-
L-tryptophan tryptophan hydroxylase form I Rattus norvegicus
0.125
-
2-amino-4-hydroxy-6-methyltetrahydropteridine tryptohan hydroxylase form I Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for maximal activity Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
4 * 59000, tryptophan hydroxylase form I from brain stem, SDS-PAGE Rattus norvegicus
288000
-
tryptophan hydroxylase form I, gradient PAGE Rattus norvegicus
300000
-
tryptophan hydroxylase form I, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan + tetrahydropteridine + O2 Rattus norvegicus enzyme catalyzes the rate-limiting step in biosynthesis of putative neurotransmitter 5-hydroxytryptamine, serotonin 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain stem Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.082
-
tryptophan hydroxylase form II Rattus norvegicus
0.374
-
brain stem, tryptophan hydroxylase form I Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, 1 month, 20% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydropteridine + O2 tryptophan hydroxylase form I, 39% of activity with L-tryptophan Rattus norvegicus tyrosine + dihydropteridine + H2O
-
?
L-tryptophan + 2-amino-4-hydroxy-6-methyl-tetrahydropteridine + O2
-
Rattus norvegicus ?
-
r
L-tryptophan + tetrahydropteridine + O2 cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine Rattus norvegicus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
?
L-tryptophan + tetrahydropteridine + O2 enzyme catalyzes the rate-limiting step in biosynthesis of putative neurotransmitter 5-hydroxytryptamine, serotonin Rattus norvegicus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 59000, tryptophan hydroxylase form I from brain stem, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
tryptophan hydroxylase form I Rattus norvegicus