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Literature summary for 1.14.16.4 extracted from

  • Fujisawa, H.; Nakata, H.
    Tryptophan 5-monooxygenase from mouse mastocytoma clone P815 (1987), Methods Enzymol., 142, 93-96.
    View publication on PubMed
Search for more literature for 1.14.16.4

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol or other sulfhydryl reagents like 2-mercaptoethanol and L-cysteine are essential for activation, 5fold increase in activity Mus musculus
L-cysteine
-
 Mus musculus

General Stability

General Stability Organism
EDTA: 0.05 mM stabilizes Mus musculus
ethylene glycol, 50%, stabilizes Mus musculus
NaCl: 1 M, in addition to other stabilizing agents brings a great improvement in stabilization Mus musculus
Tween 20: 0.06% stabilizes Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Mus musculus
2,2'-dipyridyl
-
 Mus musculus
4-Fluorotryptophan
-
 Mus musculus
5-Fluorotryptophan
-
 Mus musculus
6-Fluorotryptophan
-
 Mus musculus
8-hydroxyquinoline
-
 Mus musculus
L-p-Chlorophenylalanine
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 L-tryptophan cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine Mus musculus
0.055
-
 2-amino-4-hydroxy-6-methyltetrahydropteridine substrate L-tryptophan Mus musculus
0.1
-
 L-phenylalanine cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 5fold increase in activity Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 4 * 53000, enzyme from mastocytoma cells, SDS-PAGE Mus musculus
270000
-
 enzyme from mastocytoma cells, gel filtration Mus musculus
280000
-
 enzyme from mastocytoma cells, gradient PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan + tetrahydropteridine + O2 Mus musculus believed to be the rate-limeting enzyme in the biosynthesis of serotonin in the central nervous system 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine-agarose Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
mastocytoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.28
-
 purified from mastocytoma cells Mus musculus

Storage Stability

Storage Stability Organism
-80°C, 1 month, 30% loss of activity Mus musculus
4°C, 0.06% Tween 20, 0.05 mM EDTA, 50% ethylene glycol, 1 M NaCl, 5 days, no loss of activity Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydropteridine + O2 at a comparable rate of L-tryptophan hydroxylation Mus musculus tyrosine + dihydropteridine + H2O
-
 ?
L-tryptophan + 2-amino-4-hydroxy-6-methyl-tetrahydropteridine + O2
-
 Mus musculus ?
-
 r
L-tryptophan + tetrahydropteridine + O2 cofactor 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine Mus musculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?
L-tryptophan + tetrahydropteridine + O2 believed to be the rate-limeting enzyme in the biosynthesis of serotonin in the central nervous system Mus musculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?
L-tyrosine + tetrahydropteridine + O2 1% of activity with L-tryptophan Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 53000, enzyme from mastocytoma cells, SDS-PAGE Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 complete loss of activity in absence of stabilizing agents after 20 h Mus musculus