Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.16.4 extracted from

  • Nukiwa, T.; Tohyama, C.; Okita, C.; Kataoka, T.; Ichiyama, A.
    Purification and some properties of bovine pineal tryptophan 5-monooxygenase (1974), Biochem. Biophys. Res. Commun., 60, 1029-1035.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
6-Fluorotryptophan 1 mM, 90% inhibition of pineal tryptophan hydroxylase Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
L-tryptophan enzyme from pineal gland, cofactor tetrahydrobiopterin Bos taurus
0.032
-
L-phenylalanine enzyme from pineal gland, cofactor tetrahydrobiopterin Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
enzyme from pineal gland, gel filtration, sucrose density gradient ultracentrifugation Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme from pineal gland, ammonium sulfate, hydroxylapatite, P-cellulose, DEAE-cellulose, Sephadex G-200 Bos taurus

Reaction

Reaction Comment Organism Reaction ID
L-tryptophan + a 5,6,7,8-tetrahydropteridine + O2 = 5-hydroxy-L-tryptophan + a 4a-hydroxy-5,6,7,8-tetrahydropteridine phenylalanine hydroxylase, EC 1.14.16.1 also catalyzes this reaction Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
pineal gland
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.235
-
tryptophan hydroxylase from pineal gland Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydropteridine + O2 at a comparable rate of L-tryptophan hydroxylation Bos taurus tyrosine + dihydropteridine + H2O
-
?
L-tryptophan + tetrahydropteridine + O2
-
Bos taurus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
?