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Literature summary for 1.14.16.4 extracted from

  • Rahman, M.S.; Thomas, P.
    Molecular cloning, characterization and expression of two tryptophan hydroxylase (TPH-1 and TPH-2) genes in the hypothalamus of Atlantic croaker: down-regulation after chronic exposure to hypoxia (2009), Neuroscience, 158, 751-765.
    View publication on PubMed
Search for more literature for 1.14.16.4

Cloned(Commentary)

Cloned (Comment) Organism
PCR products ligated and cloned into pGEM-T easy vector Micropogonias undulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 sequence analysis Micropogonias undulatus
56000
-
 sequence analysis Micropogonias undulatus

Organism

Organism UniProt Comment Textmining
Micropogonias undulatus B3VBU9
-
-
Micropogonias undulatus B3VBV0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain high expression of TPH-1 throughout the brain but is greatest in the hypothalamic region Micropogonias undulatus
-
brain high expression of TPH-2 throughout the brain but it is greatest in the hypothalamic region Micropogonias undulatus
-
eye high expression of TPH-1 Micropogonias undulatus
-
eye high expression of TPH-2 Micropogonias undulatus
-
gill low levels of TPH-1 Micropogonias undulatus
-
gill low levels of TPH-2 Micropogonias undulatus
-
heart low levels of TPH-2 Micropogonias undulatus
-
kidney low levels of TPH-2 Micropogonias undulatus
-
liver low levels of TPH-2 Micropogonias undulatus
-
additional information TPH-1 is absent from heart, intestine kidney, muscle, and liver Micropogonias undulatus
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additional information TPH-2 is absent from intestine and muscle Micropogonias undulatus
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ovary low levels of TPH-1 Micropogonias undulatus
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ovary low levels of TPH-2 Micropogonias undulatus
-
testis low levels of TPH-1 Micropogonias undulatus
-
testis low levels of TPH-2 Micropogonias undulatus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + tetrahydrobiopterin + O2
-
 Micropogonias undulatus 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?

Synonyms

Synonyms Comment Organism
TPH-1
-
 Micropogonias undulatus
TPH-2
-
 Micropogonias undulatus
tryptophan hydroxylase
-
 Micropogonias undulatus

Cofactor

Cofactor Comment Organism Structure
tetrahydrobiopterin
-
 Micropogonias undulatus

Expression

Organism Comment Expression
Micropogonias undulatus TPH-1 mRNA levels are significantly decreased in the hypothalami of croaker exposed for 2 weeks to hypoxic conditions compared with those in fish exposed to normoxic conditions. Hypoxia causes a marked decline in hypothalamic TPH protein levels, which is associated with a 65% decrease in hypothalamic TPH enzyme activity and a 60% decrease in 5-hydroxytryptophan levels down
Micropogonias undulatus TPH-2 mRNA levels are significantly decreased in the hypothalami of croaker exposed for 2 weeks to hypoxic conditions compared with those in fish exposed to normoxic conditions. Hypoxia causes a marked decline in hypothalamic TPH protein levels, which is associated with a 65% decrease in hypothalamic TPH enzyme activity and a 60% decrease in 5-hydroxytryptophan levels down
Micropogonias undulatus TPH-1 mRNA is highly expressed in the total RNA extracted from the preoptic anterior hypothalamus and midbrain tegmentum compared with other brain areas up
Micropogonias undulatus TPH-2 mRNA shows high expression in the preoptic anterior hypothalamus, midbrain tegmentum and cerebellum with optic tectum, compared with other brain areas up

General Information

General Information Comment Organism
physiological function TPH is a major site of hypoxia-induced down-regulation of serotonergic function in croaker brains Micropogonias undulatus