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Literature summary for 1.14.16.4 extracted from

  • Kuhn, D.M.; Sykes, C.E.; Geddes, T.J.; Jaunarajs, K.L.; Bishop, C.
    Tryptophan hydroxylase 2 aggregates through disulfide cross-linking upon oxidation: possible link to serotonin deficits and non-motor symptoms in Parkinsons disease (2011), J. Neurochem., 116, 426-437.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.16.4

Cloned(Commentary)

Cloned (Comment) Organism
glutathione S-transferase-fusion protein expressed in Escherichia coli, expressed in HEK293-cell, eGFP-fusion protein expressed in HEK293-cell Mus musculus

Protein Variants

Protein Variants Comment Organism
C110S/C112S all cysteine residues of the regulatory domain mutated Mus musculus
C110S/C112S/C162S/C234S/C248S/C296S six N-terminal-most cysteines mutated Mus musculus
C110S/C112S/C162S/C234S/C248S/C296S/C315S/C355S/C365S/C394S/C404S/C408S/C476S all 13 cysteine residues mutated Mus musculus
C110S/C162S/C234S/C248S/C296S/C355S/C365S/C394S/C404S/C408S/C476S mutant protein containing a single cysteine residue within the regulatory domain (Cys112) and within the catalytic core domain (Cys315) Mus musculus
C162S/C234S/C248S/C296S/C315S/C355S/C365S/C394S/C404S/C408S/C476S all cysteine residues of the catalytic core domain mutated Mus musculus
C315S/C355S/C365S/C394S/C404S/C408S/C476S seven C-terminal-most cysteines mutated Mus musculus
C476S residue of the tetramerization domain Mus musculus
DELTA-C-terminal 24 residues removed tetramerization domain Mus musculus
DELTA1-40 simulates tyrosine hydroxylase 1 Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
additional information oxidation inhibits activity and leads to the formation of high molecular weight aggregates in a dithiothreitol-reversible manner, only cysteine-less mutants are resistant to this effect Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 4 * 56000, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified and cleaved from its fusion tag Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + tetrahydrobiopterin
-
 Mus musculus 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 56000, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
tryptophan hydroxylase 2
-
 Mus musculus

General Information

General Information Comment Organism
metabolism rate-limiting enzyme in the serotonin biosynthesis Mus musculus