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Literature summary for 1.14.16.5 extracted from

  • Werner, E.R.; Hermetter, A.; Prast, H.; Golderer, G.; Werner-Felmayer, G.
    Widespread occurrence of glyceryl ether monooxygenase activity in rat tissues detected by a novel assay (2007), J. Lipid Res., 48, 1422-1427.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
(6R)-tetrahydrobiopterin pH 8.5, 37°C Rattus norvegicus
0.0089
-
1-O-pyrenedecyl-sn-glycerol pH 8.5, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-alkyl-sn-glycerol + tetrahydrobiopterin + O2 Rattus norvegicus
-
1-hydroxyalkyl-sn-glycerol + dihydrobiopterin + H2O
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
Sprague-Dawley rats
-

Purification (Commentary)

Purification (Comment) Organism
partial, 100fold purification of the native enzyme from liver microsomes Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
1-O-alkyl-sn-glycerol + a 5,6,7,8-tetrahydropteridine + O2 = 1-O-(1-hydroxyalkyl)-sn-glycerol + a 4a-hydroxy-5,6,7,8-tetrahydropteridine The glyceryl ether substrate is first hydroxylated in a pteridine-dependent monooxygenase reaction to yield the unstable semiacetal product. The enzyme accepts a range of chain lengths from n = 11 to n = 20. The tetrahydrobiopterin cofactor is first converted to a 4alpha-hydroxy derivative and leaves the reaction after dehydration as 6,7[8H]-dihydrobiopterin, which is recycled to tetrahydrobiopterin by dihydropteridine reductase with NADH or NADPH. The semiacetal rearranges to the corresponding aldehyde and glycerol. In the presence of oxygen, the aldehyde is readily oxidized to the corresponding acid Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-
cerebellum
-
Rattus norvegicus
-
cerebral cortex
-
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
large intestine
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
lung
-
Rattus norvegicus
-
additional information wide tissue distribution, except female heart, with highest activities in liver, intestine, and cerebellum, overview Rattus norvegicus
-
ovary
-
Rattus norvegicus
-
skeletal muscle
-
Rattus norvegicus
-
small intestine
-
Rattus norvegicus
-
spleen
-
Rattus norvegicus
-
stomach
-
Rattus norvegicus
-
striatum
-
Rattus norvegicus
-
testis
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-alkyl-sn-glycerol + tetrahydrobiopterin + O2
-
Rattus norvegicus 1-hydroxyalkyl-sn-glycerol + dihydrobiopterin + H2O
-
?
1-O-pyrenedecyl-sn-glycerol + tetrahydrobiopterin + O2 1-O-pyrenedecyl-sn-glycerol and high-performance liquid chromatographic analysis of reaction mixtures with fluorescence detection, overview Rattus norvegicus 1-hydroxypyrenedecyl-sn-glycerol + dihydrobiopterin + H2O further formation of 1-pyrenedecanoic acid ?

Synonyms

Synonyms Comment Organism
glyceryl ether monooxygenase
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
(6R)-tetrahydrobiopterin strictly dependent on, stereospecific, overview Rattus norvegicus
(6RS)-6,7-dimethyltetrahydropterin 94.2% of the activity with 6R-tetrahydrobiopterin Rattus norvegicus
(6RS)-6-hydroxymethyltetrahydropterin 16% of the activity with 6R-tetrahydrobiopterin Rattus norvegicus
(6RS)-6-methyltetrahydropterin 60.9% of the activity with 6R-tetrahydrobiopterin Rattus norvegicus
(6RS)-tetrahydrodictyopterin 44.7% of the activity with 6R-tetrahydrobiopterin Rattus norvegicus
(6RS)-tetrahydroneopterin 124% of the activity with 6R-tetrahydrobiopterin Rattus norvegicus
(6S)-tetrahydrobiopterin 3.5% of the activity with 6R-tetrahydrobiopterin Rattus norvegicus