Literature summary for 1.14.17.1 extracted from

Long, R.A.; Weppelman, R.M.; Taylor, J.E.; Tolman, R.L.; Olson, G.
Purification and characterization of avian dopamine beta-hydroxylase (1981), Biochemistry, 20, 7423-7431.

Search for more literature for 1.14.17.1

Activating Compound

Activating Compound	Comment	Organism	Structure
acetate	-	Gallus gallus
dehydroascorbate	-	Gallus gallus
fumarate	-	Gallus gallus

Inhibitors

Inhibitors	Comment	Organism	Structure
ascorbate	-	Gallus gallus
norepinephrine	-	Gallus gallus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2	-	dopamine	-	Gallus gallus
2	-	ascorbate	-	Gallus gallus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chromaffin granule	-	Gallus gallus	42583	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
NaCl	-	Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	4 * 80000, SDS-PAGE after treatment with 2-mercaptoethanol, subunits joined in pairs by disulfide bonds	Gallus gallus
316000	-	gel filtration	Gallus gallus

Organism

Organism	UniProt	Comment	Textmining
Gallus gallus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Gallus gallus

Purification (Commentary)

Purification (Comment)	Organism
-	Gallus gallus

Reaction

Reaction	Comment	Organism	Reaction ID
dopamine + 2 ascorbate + O2 = noradrenaline + 2 monodehydroascorbate + H2O	uni-uni bi-uni ping pong mechanism	Gallus gallus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
adrenal gland	-	Gallus gallus	-

Storage Stability

Storage Stability	Organism
-20°C, stable for at least 6 weeks	Gallus gallus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3,4-dihydroxyphenethylamine + ascorbate + O2	3,4-dihydroxyphenethylamine is identical with 4-(2-aminoethyl)-1,2-benzendiol i.e. dopamine, ferricyanide can replace ascorbate	Gallus gallus	noradrenaline + dehydroascorbate + H2O	norepinephrine	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 80000, SDS-PAGE after treatment with 2-mercaptoethanol, subunits joined in pairs by disulfide bonds	Gallus gallus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
-	4	24 h, 10-15% loss of activity	Gallus gallus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	6	-	Gallus gallus

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.5	6.5	sharp decrease in activity between pH 4.5 and 5.0 and between pH 6.0 and 6.5	Gallus gallus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
5	-	norepinephrine	-	Gallus gallus
20	-	ascorbate	-	Gallus gallus

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Release 2023.1 (January 2023)