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Literature summary for 1.14.17.3 extracted from
- Characterization of a half-apo derivative of peptidylglycine monooxygenase. Insight into the reactivity of each active site copper (2001), Biochemistry, 40, 6867-6875.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| medullary thyroid alpha-AE, type A, expression in chinese hamster ovary cells | Rattus norvegicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | Cu2+ to protein ration is 0.97 | Rattus norvegicus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| peptidylglycine + ascorbate + O2 | Rattus norvegicus | - |
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
bifunctional alpha-AE | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| type A alpha-AE, recombinant from chinese hamster ovary cells | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| [peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O | structural analysis | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| medullary thyroid carcinoma cell | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
| 5.9 | - |
purified recombinant enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-N-acetyl-Tyr-Val-Gly + ascorbate + O2 | - |
Rattus norvegicus | alpha-N-acetyl-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
| dansyl-D-Tyr-Val-Gly + ascorbate + O2 | - |
Rattus norvegicus | dansyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
| peptidylglycine + ascorbate + O2 | - |
Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
| peptidylglycine + ascorbate + O2 | requirement for O2 | Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
| peptidylglycine + ascorbate + O2 | COOH-terminal glycine | Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the 2-step process is catalyzed by only 1 enzyme called type A peptidylglycine alpha-amidating enzyme | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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