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Literature summary for 1.14.17.B1 extracted from

  • Loeffler, S.; Zenk, M.H.
    The hydroxylation step in the biosynthetic pathway leading from norcolaurine to reticuline (1990), Phytochemistry, 29, 3499-3503.
No PubMed abstract available
Search for more literature for 1.14.17.B1

Inhibitors

Inhibitors Comment Organism Structure
azide 12 mM, 50% inhibition Berberis stolonifera
Bathocuproine
-
 Berberis stolonifera
CN- 1 mM, complete inhibition Berberis stolonifera
diethyldithiocarbamate 0.3 mM, complete inhibition Berberis stolonifera
dipicolinic acid
-
 Berberis stolonifera
EDTA no inhibition EDTA Berberis stolonifera
Thiourea 13 mM, 50% inhibition Berberis stolonifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 36000, SDS-PAGE Berberis stolonifera
60000
-
 gel filtration Berberis stolonifera

Organism

Organism UniProt Comment Textmining
Berberis stolonifera
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sephadex G-25, hydoroxylapatite-DEAE-Sephacel, Q Sepharose, Mono Q, isoelectric focusing Berberis stolonifera

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.065
-
 with tyrosine Berberis stolonifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-coclaurine + ascorbate + O2
-
 Berberis stolonifera (R)-3-hydroxycoclaurine + dehydroascorbate + H2O
-
 ?
(S)-coclaurine + ascorbate + O2
-
 Berberis stolonifera (S)-3-hydroxycoclaurine + dehydroascorbate + H2O
-
 ?
4-methylcatechol + ascorbate + O2
-
 Berberis stolonifera 4-methyl-o-benzoquinone + dehydroascorbate + H2O
-
 ?
L-tyrosine + ascorbate + O2
-
 Berberis stolonifera L-dopa + dehydroascorbate + H2O
-
 ?
N-methylcoclaurine + ascorbate + O2
-
 Berberis stolonifera 3-hydroxy-N-methylcoclaurine + dehydroascorbate + H2O
-
 ?
tyramine + ascorbate + O2
-
 Berberis stolonifera 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + dehydroascorbate + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE Berberis stolonifera

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 30
-
 Berberis stolonifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Berberis stolonifera

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7.5 50% of maximal activity at pH 4.5 and pH 7.5 Berberis stolonifera