Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one | - |
Streptomyces sp. | |
arbutin | no inhibition by 1 mM arbutin | Streptomyces sp. | |
azide | no inhibition by sodium azide | Streptomyces sp. | |
diethyldithiocarbamate | - |
Streptomyces sp. | |
DL-dithiothreitol | - |
Streptomyces sp. | |
EDTA | no inhibition EDTA | Streptomyces sp. | |
ethanol | 50% ethanol, 56% inhibition | Streptomyces sp. | |
L-cysteine | - |
Streptomyces sp. | |
methanol | 50% inhibition in 50% methanol | Streptomyces sp. | |
additional information | not inhibited by 1 mM arbutin, or 0.2 mM Cu2+, Fe3+, Zn2+, Mg2+ and Ca2+ | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.25 | - |
L-tyrosine | - |
Streptomyces sp. | |
1.64 | - |
DL-tyrosine | - |
Streptomyces sp. | |
1.98 | - |
D-tyrosine | - |
Streptomyces sp. | |
4.14 | - |
L-Dopa | - |
Streptomyces sp. | |
9.56 | - |
DL-dopa | - |
Streptomyces sp. | |
28.7 | - |
D-Dopa | - |
Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | Streptomyces sp. |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | no loss of activity after 20 h at 30°C in 20% acetone | Streptomyces sp. |
dimethyl sulfoxide | no loss of activity after 20 h at 30°C in 20% dimethylsulfoxide | Streptomyces sp. |
Ethanol | 30% ethanol, 30°C, pH 7.0, 17% loss of activity after 20 h | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mushroom | - |
- |
- |
Streptomyces sp. | - |
REN-21, organic solvent resistant enzyme | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-cellulose, QAE-Sephadex A-50, Toyopearl HW-55 | Streptomyces sp. |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
130 | - |
- |
Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-dopa + 1/2 O2 | - |
Streptomyces sp. | dopaquinone + H2O | - |
r | |
D-dopa + O2 | - |
Streptomyces sp. | dopaquinone + H2O | - |
r | |
D-tyrosine + O2 | - |
Streptomyces sp. | dopaquinone + H2O | - |
r | |
D-tyrosine + O2 | - |
Streptomyces sp. | ? | - |
r | |
DL-dopa + 1/2 O2 | - |
Streptomyces sp. | dopaquinone + H2O | - |
r | |
DL-DOPA + O2 | - |
Streptomyces sp. | dopaquinone + H2O | - |
r | |
DL-tyrosine + O2 | - |
Streptomyces sp. | dopaquinone + H2O | - |
r | |
L-epicatechin + O2 | 155% of activity with L-dopa | Streptomyces sp. | ? | - |
? | |
L-tyrosine + O2 + AH2 | - |
Streptomyces sp. | L-dopa + H2O + A | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Mushroom |
? | x * 32000, SDS-PAGE | Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
with L-dopa | Streptomyces sp. |
40 | - |
- |
Mushroom |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.27 | - |
D-tyrosine | - |
Streptomyces sp. | |
74.2 | - |
DL-tyrosine | - |
Streptomyces sp. | |
81.4 | - |
L-tyrosine | - |
Streptomyces sp. | |
117 | - |
D-Dopa | - |
Streptomyces sp. | |
1120 | - |
L-Dopa | - |
Streptomyces sp. | |
1170 | - |
DL-dopa | - |
Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
with L-dopa | Streptomyces sp. |