Cloned (Comment) | Organism |
---|---|
the cDNAs encoding three red clover PPOs are expressed individually in alfalfa (Medicago sativa L.) | Trifolium pratense |
the cDNAs encoding three Trifolium pratensePPOs are expressed individually in alfalfa (Medicago sativa L.) | Trifolium pratense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
several minor bands (50, 37, and 35 kDa) are observed in PPO2-expressed in alfalfa | Trifolium pratense |
44000 | 47000 | two protein bands (approximately 44-47 kDa) appear in transgenic alfalfa expressing PPO3 | Trifolium pratense |
45000 | - |
by 1-2 days of incubation, an additional protein band of approximately 45 kDa becomes visible in extracts of red clover and transgenic alfalfa expressing PPO1 or PPO2 | Trifolium pratense |
65000 | - |
PPOs expressed in transgenic alfalfa and Trifolium pratense | Trifolium pratense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trifolium pratense | Q84YH9 | PPO3, polyphenol oxidase (EC 1.10.3.1) (EC 1.14.18.1) | - |
Trifolium pratense | Q84YI0 | PPO2, polyphenol oxidase (EC 1.10.3.1) (EC 1.14.18.1) | - |
Trifolium pratense | Q84YI1 | PPO1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0204 | - |
values shown are normalized to the specific activity (in nmol/s mg) of each PPO for caffeic acid (LtPPO2: 0.34) | Trifolium pratense |
0.09 | - |
values shown are normalized to the specific activity of each PPO for caffeic acid (LtPPO3: 1.5 nmol/s mg) | Trifolium pratense |
0.216 | - |
values shown are normalized to the specific activity of each PPO for caffeic acid (LtPPO1: 3.6 nmol/s mg) | Trifolium pratense |
0.348 | - |
values shown are normalized to the specific activity (in nmol/s mg) of each PPO for caffeic acid (AcPPO2: 5.8) | Trifolium pratense |
4.08 | - |
values shown are normalized to the specific activity (in nmol/s mg) of each PPO for caffeic acid (AcPPO3, 68 nmol/s mg) | Trifolium pratense |
5.94 | - |
values shown are normalized to the specific activity (in nmol/s mg) of each PPO for caffeic acid (AcPPO1: 99 nmol/s mg) | Trifolium pratense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-catechin + 1/2 O2 | - |
Trifolium pratense | ? | - |
? | |
(-)-epicatechin + 1/2 O2 | - |
Trifolium pratense | ? | - |
? | |
caffeic acid + 1/2 O2 | - |
Trifolium pratense | caffeoyl quinone + H2O | - |
? | |
catechol + 1/2 O2 | - |
Trifolium pratense | 1,2-benzoquinone + H2O | - |
? | |
chlorogenic acid + 1/2 O2 | - |
Trifolium pratense | chlorogenoquinone + H2O | - |
? | |
dopamine + 1/2 O2 | - |
Trifolium pratense | dopaminequinone + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
polyphenol oxidase | - |
Trifolium pratense |
PPO | - |
Trifolium pratense |
PPO1 | - |
Trifolium pratense |
PPO2 | - |
Trifolium pratense |
PPO3 | - |
Trifolium pratense |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
PPO1 appears to be more stable at high temperatures than PPO2 or PPO3 | Trifolium pratense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.1 | - |
for PPO3, activation of the PPOs shifts the pH optima to 7 | Trifolium pratense |
5.5 | - |
for PPO1, activation of the PPOs shifts the pH optima to 7 | Trifolium pratense |
6.9 | - |
for PPO2, activation of the PPOs shifts the pH optima to 7 | Trifolium pratense |