Any feedback?
Literature summary for 1.14.18.1 extracted from
- Constituents from the Formosan apple reduce tyrosinase activity in human epidermal melanocytes (2007), Phytochemistry, 68, 1189-1199.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | 3-hydroxyphloretin and catechol isolated from the formosan apple, may be useful as cosmetic agents to stimulate skin whitening | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-hydroxyphloretin | constituents from the formosan apple (Malus doumeri var. formosana), exhibits a dose-dependent inhibitory effect on mushroom tyrosinase activity, competitive inhibitor. Enzyme kinetics study of 3-hydroxyphloretin as inhibitor with various concentrations of the L-tyrosine substrate (15.625, 31.25, 62.5, 125, 250, 500 microM) | Agaricus bisporus |  |
| 3-hydroxyphloretin | constituents from the formosan apple (Malus doumeri var. formosana), inhibition 73%, shows substantial cellular tyrosinase inhibitory activity at a concentration of 100 microM | Homo sapiens | |
| 3-hydroxyphloridzin | - |
Homo sapiens | |
| arbutin | commercially available depigmenting agent, used as a positive control | Homo sapiens | |
| ascorbic acid | exhibits less tyrosinase-inhibitory activity than 3-hydroxyphloretin or catechol | Homo sapiens | |
| catechol | constituents from the formosan apple (Malus doumeri var. formosana) | Agaricus bisporus | |
| catechol | constituents from the formosan apple (Malus doumeri var. formosana), inhibition 78%, shows substantial cellular tyrosinase inhibitory activity at a concentration of 100 microM | Homo sapiens | |
| chrysin | - |
Homo sapiens | |
| ellagic acid | exhibits less tyrosinase-inhibitory activity than 3-hydroxyphloretin or catechol | Homo sapiens | |
| hydroquinone | exhibits less tyrosinase-inhibitory activity than 3-hydroxyphloretin or catechol, very toxic to HEMn cell | Homo sapiens | |
| phloretin | - |
Homo sapiens | |
| phloridzin | - |
Homo sapiens | |
| pinosylvin | inhibition of 32% | Homo sapiens | |
| protocatechuic acid | inhibition of 34% | Homo sapiens | |
| quercetin | inhibition of 36% | Homo sapiens | |
| rutin | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.39 | - |
L-tyrosine | - |
Agaricus bisporus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agaricus bisporus | - |
- |
- |
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HEMn cell | - |
Homo sapiens | - |
| melanocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
mushroom tyrosinase purified enzyme assay with 5000 U/ml | Agaricus bisporus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-dopa + 1/2 O2 | - |
Homo sapiens | L-dopaquinone + H2O | - |
? | |
| L-tyrosine + O2 + AH2 | - |
Agaricus bisporus | L-dopa + H2O + A | - |
? | |
| tyrosine + O2 + AH2 | - |
Homo sapiens | L-dopa + H2O + A | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tyrosinase | - |
Homo sapiens |
| tyrosinase | - |
Agaricus bisporus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
potent hydroxyl radical-scavenging, phloretin, quercetin, 3-hydroxyphloretin, catechol, and pinosylvin have the strongest hydroxyl radical-scavenging properties | Homo sapiens | 3-hydroxyphloretin | |
| 0.0008 | - |
the effect of the formosan apple constituents on hydroxyl radical-scavenging activity. Phloretin, quercetin, 3-hydroxyphloretin, catechol, and pinosylvin have the strongest hydroxyl radical-scavenging properties | Homo sapiens | phloretin | |
| 0.0011 | - |
potent hydroxyl radical-scavenging, phloretin, quercetin, 3-hydroxyphloretin, catechol, and pinosylvin have the strongest hydroxyl radical-scavenging properties | Homo sapiens | catechol | |
| 0.0012 | - |
the effect of the formosan apple constituents on hydroxyl radical-scavenging activity, phloretin, quercetin, 3-hydroxyphloretin, catechol, and pinosylvin have the strongest hydroxyl radical-scavenging properties | Homo sapiens | quercetin | |
| 0.0025 | - |
the effect of the formosan apple constituents on hydroxyl radical-scavenging activity, phloretin, quercetin, 3-hydroxyphloretin, catechol, and pinosylvin have the strongest hydroxyl radical-scavenging properties | Homo sapiens | pinosylvin | |
| 0.022 | - |
cellular tyrosinase-reducing activities, reduction of tyrosinase in HEMn cells | Homo sapiens | catechol | |
| 0.03 | - |
the effect of the formosan apple constituents on hydroxyl radical-scavenging activity | Homo sapiens | 3-hydroxyphlorizin | |
| 0.032 | - |
cellular tyrosinase-reducing activities, reduction of tyrosinase in HEMn cells | Homo sapiens | 3-hydroxyphloretin | |
| 0.042 | - |
the effect of the formosan apple constituents on hydroxyl radical-scavenging activity | Homo sapiens | protocatechuic acid | |
| 0.045 | - |
the effect of the formosan apple constituents on hydroxyl radical-scavenging activity | Homo sapiens | chrysin | |
| 0.0634 | - |
exhibits a dose-dependent inhibitory effect on mushroom tyrosinase activity, enzyme kinetics study of 3-hydroxyphloretin as inhibitor with various concentrations of the L-tyrosine substrate (15.625, 31.25, 62.5, 125, 250, 500 microM) | Agaricus bisporus | 3-hydroxyphloretin | |
| 0.08 | - |
the effect of the formosan apple constituents on hydroxyl radical-scavenging activity | Homo sapiens | rutin | |
| 0.087 | - |
the effect of the formosan apple constituents on hydroxyl radical-scavenging activity | Homo sapiens | phloridzin | |
| 3 | - |
- |
Homo sapiens | arbutin | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
