Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Priestia megaterium |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
R209H | the mutant possesses a higher monophenolase/diphenolase activity ratio than the wild type enzyme | Priestia megaterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
kojic acid | i.e. 5-hydroxy-2-(hydroxymethyl)-g-pyrone | Priestia megaterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | tyrosinase is a member of the type 3 copper enzyme family, two Cu2+ ions serveas the major cofactors within the active site | Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, X-ray crystallography | Priestia megaterium |
70000 | - |
gel filtration | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | B2ZB02 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + O2 | - |
Priestia megaterium | dopachrome + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 35000, X-ray crystallography | Priestia megaterium |
Synonyms | Comment | Organism |
---|---|---|
tyr | - |
Priestia megaterium |
tyrosinase | - |
Priestia megaterium |