Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.18.2 extracted from

  • Muchmore, E.A.; Milewski, M.; Varki, A.; Diaz, S.
    Biosynthesis of N-glycolyneuraminic acid. The primary site of hydroxylation of N-acetylneuraminic acid is the cytosolic sugar nucleotide pool (1989), J. Biol. Chem., 264, 20216-20223.
    View publication on PubMed
Search for more literature for 1.14.18.2

General Stability

General Stability Organism
10 mM in the initial homogenization buffer is required for stability during 60-90 min incubation Mus musculus
10 mM in the initial homogenization buffer is required for stability during 60-90 min incubation Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
3'-AMP 5 mM, complete inhibition Sus scrofa
3'-CMP 5 mM, about 20% inhibition Sus scrofa
3'-UMP 5 mM, about 90% inhibition Sus scrofa
5'-AMP 5 mM, about 78% inhibition Sus scrofa
5'-UMP 5 mM, about 15% inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6 0.9 CMP-N-acetylneuraminate pH 7.4, 37°C, enzyme from NS-1 cells Mus musculus
2.5
-
 CMP-N-acetylneuraminate pH 7.4, 37°C, enzyme from submaxillary gland Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mus musculus 5829
-
cytosol
-
 Sus scrofa 5829
-

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Mus musculus
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
colonic mucosa
-
 Rattus norvegicus
-
P3/NS1/1-Ag4.1 cell
-
 Mus musculus
-
P3X63Ag8 cell
-
 Mus musculus
-
submaxillary gland
-
 Sus scrofa
-
submaxillary gland
-
 Bos taurus
-

Storage Stability

Storage Stability Organism
-70°C, stable in crude extract for several weeks Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity towards free or alpha-glycosidically bound N-acetylneuraminic acid Mus musculus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 7.4 enzyme preparation Mus musculus
6.4 7.4 enzyme preparation Sus scrofa