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Literature summary for 1.14.18.3 extracted from

  • Zahn, J.A.; DiSpirito, A.A.
    Membrane-associated methane monooxygenase from Methylococcus capsulatus (Bath) (1996), J. Bacteriol., 178, 1018-1029.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.18.3

Activating Compound

Activating Compound Comment Organism Structure
Cu2+ pMMO, optimal at 0.3 mM Methylococcus capsulatus
Fe3+ pMMO, optimal at 5.0 mM Methylococcus capsulatus

Inhibitors

Inhibitors Comment Organism Structure
2-Heptyl-4-hydroxyquinoline-N-oxide pMMO, at 0.05 mM Methylococcus capsulatus
Acetylene pMMO Methylococcus capsulatus
Cu+ pMMO Methylococcus capsulatus
cysteine pMMO Methylococcus capsulatus
Fe2+ slightly, membrane-bound enzyme form Methylococcus capsulatus
KCN pMMO Methylococcus capsulatus
Myxothiazol pMMO, suicide substrate Methylococcus capsulatus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm cytoplasmatic, soluble enzyme form termed sMMO Methylococcus capsulatus 5737
-
membrane membrane-bound particulate enzyme form termed pMMO Methylococcus capsulatus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 14.5 atoms per molecule of enzyme pMMO, type II copper centre Methylococcus capsulatus
Iron 2.5 atoms per enzyme molecule of pMMO Methylococcus capsulatus
Mn2+ pMMO, low content Methylococcus capsulatus
Mo2+ pMMO, low content Methylococcus capsulatus
additional information copper-induced iron-uptake Methylococcus capsulatus
Zn2+ pMMO Methylococcus capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 1 * 47000 + 1 * 27000 + 1 * 25000, pMMO, mass spectrometry and SDS-PAGE Methylococcus capsulatus
27000
-
 1 * 47000 + 1 * 27000 + 1 * 25000, pMMO, mass spectrometry and SDS-PAGE Methylococcus capsulatus
47000
-
 1 * 47000 + 1 * 27000 + 1 * 25000, pMMO, mass spectrometry and SDS-PAGE Methylococcus capsulatus
99000
-
 pMMO, mass spectrometry Methylococcus capsulatus

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus
-
 Bath
-
Methylococcus capsulatus
-
 enzyme form pMMO is copper-inducible
-
Methylococcus capsulatus Bath
-
 Bath
-

Purification (Commentary)

Purification (Comment) Organism
pMMO Methylococcus capsulatus

Reaction

Reaction Comment Organism Reaction ID
methane + quinol + O2 = methanol + quinone + H2O mechanism of pMMO Methylococcus capsulatus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.011
-
 purified enzyme form pMMO Methylococcus capsulatus
0.0605
-
 whole cells, substrate formate Methylococcus capsulatus

Storage Stability

Storage Stability Organism
4°C, reducing argon or nitrogen atmosphere, no loss of activity after 1 week Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus methanol + acceptor + H2O
-
 ?
methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus Bath methanol + acceptor + H2O
-
 ?
propene + reduced acceptor + H+ + O2 enzyme form sMMO Methylococcus capsulatus 1,2-epoxypropane + acceptor + H2O
-
 ?
propene + reduced acceptor + H+ + O2 enzyme form sMMO Methylococcus capsulatus Bath 1,2-epoxypropane + acceptor + H2O
-
 ?

Subunits

Subunits Comment Organism
More pMMO subunit A has acetylene binding ability Methylococcus capsulatus
trimer 1 * 47000 + 1 * 27000 + 1 * 25000, pMMO, mass spectrometry and SDS-PAGE Methylococcus capsulatus

Synonyms

Synonyms Comment Organism
MMO
-
 Methylococcus capsulatus
pMMO particulate, membrane-bound enzyme form Methylococcus capsulatus
sMMO soluble, cytoplasmic enzyme form Methylococcus capsulatus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Methylococcus capsulatus