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Literature summary for 1.14.18.3 extracted from

  • Lee, S.W.; Keeney, D.R.; Lim, D.H.; Dispirito, A.A.; Semrau, J.D.
    Mixed pollutant degradation by Methylosinus trichosporium OB3b expressing either soluble or particulate methane monooxygenase: can the tortoise beat the hare? (2006), Appl. Environ. Microbiol., 72, 7503-7509.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
degradation pMMO can be used for biodegradation of mixtures of chlorinated solvents, i.e., trichloroethylene, trans-dichloroethylene, and vinyl chloride. If the concentrations are increased to 0.1 mM, pMMO-expressing cells grow faster and degrade more of these pollutants in a shorter amount of time than sMMO Methylosinus trichosporium

Inhibitors

Inhibitors Comment Organism Structure
Acetylene complete inhibition at 0.1 mM Methylosinus trichosporium

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane intracytoplasmic membrane Methylosinus trichosporium 16020
-

Organism

Organism UniProt Comment Textmining
Methylosinus trichosporium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + trans-dichloroethylene + vinyl chloride + trichloroethylene + O2
-
Methylosinus trichosporium ?
-
?

Synonyms

Synonyms Comment Organism
particulate methane monooxygenase
-
Methylosinus trichosporium
pMMO
-
Methylosinus trichosporium

Expression

Organism Comment Expression
Methylosinus trichosporium for pMMO-expressing conditions, 0.02 mM copper is added and is equilibrated for at least 1 day before the media are inoculated up