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Literature summary for 1.14.18.3 extracted from

  • Smith, S.M.; Rawat, S.; Telser, J.; Hoffman, B.M.; Stemmler, T.L.; Rosenzweig, A.C.
    Crystal structure and characterization of particulate methane monooxygenase from Methylocystis species strain M (2011), Biochemistry, 50, 10231-10240.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M MES, pH 6.5, 18% (w/v) PEG 750 MME, 0.03 M glycyl glycine, 0.01 M ZnSO4, at 22°C Methylocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
cyanide cyanide treatment of the enzyme removes about 98% of the copper and about 49% of the iron and abolishes all activity Methylocystis sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Methylocystis sp. 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
copper the enzyme contains about 2.3 copper ions per 100 kDa protomer the enzyme contains a mixture of Cu+ and Cu2+ Methylocystis sp.
Fe2+ the enzyme contains about 0.6 Fe2+ ions per 100 kDa protomer Methylocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methane + reduced acceptor + H* + O2 Methylocystis sp.
-
methanol + acceptor + H2O
-
?

Organism

Organism UniProt Comment Textmining
Methylocystis sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0053
-
using propylene as substrate, pH and temperature not specified in the publication Methylocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + reduced acceptor + H* + O2
-
Methylocystis sp. methanol + acceptor + H2O
-
?
Mn2+ + H2O2
-
Methylocystis sp. ?
-
?

Synonyms

Synonyms Comment Organism
particulate methane monooxygenase
-
Methylocystis sp.
pMMO
-
Methylocystis sp.

Cofactor

Cofactor Comment Organism Structure
NADH
-
Methylocystis sp.