Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M MES, pH 6.5, 18% (w/v) PEG 750 MME, 0.03 M glycyl glycine, 0.01 M ZnSO4, at 22°C | Methylocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | cyanide treatment of the enzyme removes about 98% of the copper and about 49% of the iron and abolishes all activity | Methylocystis sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Methylocystis sp. | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | the enzyme contains about 2.3 copper ions per 100 kDa protomer the enzyme contains a mixture of Cu+ and Cu2+ | Methylocystis sp. | |
Fe2+ | the enzyme contains about 0.6 Fe2+ ions per 100 kDa protomer | Methylocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + reduced acceptor + H* + O2 | Methylocystis sp. | - |
methanol + acceptor + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylocystis sp. | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0053 | - |
using propylene as substrate, pH and temperature not specified in the publication | Methylocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + reduced acceptor + H* + O2 | - |
Methylocystis sp. | methanol + acceptor + H2O | - |
? | |
Mn2+ + H2O2 | - |
Methylocystis sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
particulate methane monooxygenase | - |
Methylocystis sp. |
pMMO | - |
Methylocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methylocystis sp. |