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Literature summary for 1.14.18.3 extracted from

  • Chen, K.H.; Wu, H.H.; Ke, S.F.; Rao, Y.T.; Tu, C.M.; Chen, Y.P.; Kuei, K.H.; Chen, Y.S.; Wang, V.C.; Kao, W.C.; Chan, S.I.
    Bacteriohemerythrin bolsters the activity of the particulate methane monooxygenase (pMMO) in Methylococcus capsulatus (Bath) (2012), J. Inorg. Biochem., 111, 10-17.
    View publication on PubMed
Search for more literature for 1.14.18.3

Activating Compound

Activating Compound Comment Organism Structure
bacteriohemerythrin enhances enzyme activity. The maximum activity is observed at a enzyme to bacteriohemerythrin concentration ratio of 4:1 Methylococcus capsulatus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Methylococcus capsulatus

Inhibitors

Inhibitors Comment Organism Structure
duroquinone noncompetitive inhibitor Methylococcus capsulatus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Methylococcus capsulatus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
copper the enzyme contains 13 copper ions Methylococcus capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylococcus capsulatus
27000
-
 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylococcus capsulatus
45000
-
 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylococcus capsulatus

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
MonoQ 10/100 GL column chromatography and Sephacryl S100 gel filtration Methylococcus capsulatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1037
-
 at 45°C and pH 7.0, with NADH as cosubstrate, in the presence of bacteriohemerythrin Methylococcus capsulatus
0.1228
-
 at 45°C and pH 7.0, with duroquinol as cosubstrate, in the presence of bacteriohemerythrin Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propylene + duroquinol + O2
-
 Methylococcus capsulatus propylene oxide + duroquinone + H2O
-
 ?

Subunits

Subunits Comment Organism
heterotrimer 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylococcus capsulatus

Synonyms

Synonyms Comment Organism
particulate methane monooxygenase
-
 Methylococcus capsulatus
pMMO
-
 Methylococcus capsulatus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Methylococcus capsulatus