Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Mycolicibacterium phlei | |
CN- | - |
Mycolicibacterium phlei | |
Cu+ | strong, complete inhibition at 0.1 mM | Mycolicibacterium phlei | |
dihydrolipoic acid | - |
Mycolicibacterium phlei | |
EDTA | - |
Mycolicibacterium phlei | |
menadione | 85% inhibition at 0.1 mM | Mycolicibacterium phlei | |
methylene blue | - |
Mycolicibacterium phlei | |
additional information | sulfhydryl compounds | Mycolicibacterium phlei | |
NADH | - |
Mycolicibacterium phlei | |
o-phenanthroline | 80% inhibition at 2 mM | Mycolicibacterium phlei | |
p-chloromercuribenzoate | strong, complete inhibition at 0.02 mM | Mycolicibacterium phlei | |
phenazine methosulfate | nearly complete inhibition at 0.3 mM | Mycolicibacterium phlei | |
riboflavin | 25-30% inhibition at 0.04 mM | Mycolicibacterium phlei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
FAD | - |
Mycolicibacterium phlei | |
0.0025 | - |
FMN | - |
Mycolicibacterium phlei | |
0.8 | - |
NADPH | - |
Mycolicibacterium phlei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | specifically required | Mycolicibacterium phlei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
stearoyl-CoA + AH2 + O2 | Mycolicibacterium phlei | - |
oleoyl-CoA + A + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium phlei | - |
ATCC 356 | - |
Storage Stability | Organism |
---|---|
-20°C, sedimented enzyme, 0.25 M sucrose, 2 weeks, without significant loss of activity | Mycolicibacterium phlei |
-20°C, supernatant fraction, over 1 year, without loss of activity | Mycolicibacterium phlei |
4°C, 1 mM or 10 mM potassium phosphate buffer, pH 7.2, 24 hours, activity irreversibly lost | Mycolicibacterium phlei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + NADPH + O2 | - |
Mycolicibacterium phlei | ? | - |
? | |
stearoyl-CoA + AH2 + O2 | - |
Mycolicibacterium phlei | oleoyl-CoA + A + H2O | - |
? | |
stearoyl-CoA + NADPH + O2 | - |
Mycolicibacterium phlei | oleoyl-CoA + NADP+ + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mycolicibacterium phlei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
30 min in distilled water or 0.1 M phosphate buffer, pH 7.2 causes in almost complete loss of activity | Mycolicibacterium phlei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Mycolicibacterium phlei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | required | Mycolicibacterium phlei | |
FMN | required, almost as active as FAD | Mycolicibacterium phlei | |
NADPH | specifically required, seems not to be directly involved in the desaturation reaction but serves to keep another cofactor in the reduced state, maybe FADH | Mycolicibacterium phlei |