Cloned (Comment) | Organism |
---|---|
in vitro translation using a wheat germ cell-free extract in the presence of unilamelar liposomes, method optimization of cell-free translation to the assembly and purification of integral membrane protein complexes | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | near complete transfer of the in vitro expressed enzyme integral membrane protein into the liposomes, followed by single step purification via centrifugation in a density gradient yielded proteoliposomes with the desaturase in high purity as judged by capillary electrophoresis, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | heme and non-heme iron | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
stearoyl-CoA + ferrocytochrome b5 + O2 + H+ | Homo sapiens | - |
oleoyl-CoA + ferricytochrome b5 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00767 | - |
- |
Purification (Comment) | Organism |
---|---|
near complete transfer of the in vitro expressed enzyme integral membrane protein into the liposomes, followed by single step purification via centrifugation in a density gradient yielded proteoliposomes with the desaturase in high purity as judged by capillary electrophoresis, method optimization of cell-free translation to the assembly and purification of integral membrane protein complexes, overview | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
in vitro reconstitution of the non-heme iron and heme active sites to a functional reconstituted enzyme complex after in vitro translation and protein integration into liposomes, method optimization, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
stearoyl-CoA + ferrocytochrome b5 + O2 + H+ | - |
Homo sapiens | oleoyl-CoA + ferricytochrome b5 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SCD | - |
Homo sapiens |
stearoyl-CoA desaturase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ferrocytochrome b5 | - |
Homo sapiens |