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Literature summary for 1.14.19.11 extracted from
- A multifunctional acyl-acyl carrier protein desaturase from Hedera helix L. (English ivy) can synthesize 16- and 18-carbon monoene and diene products (2005), J. Biol. Chem., 280, 28169-28176.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 DE3 Gold cells and in Arabidopsis thaliana | Hedera helix |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
palmitoyl-[acyl-carrier protein] | pH and temperature not specified in the publication | Hedera helix |  |
| 0.0015 | - |
stearoyl-[acyl-carrier protein] | pH and temperature not specified in the publication | Hedera helix | |
| 0.002 | - |
oleoyl-[acyl-carrier protein] | pH and temperature not specified in the publication | Hedera helix | |
| 0.0021 | - |
palmitoleoyl-[acyl-carrier protein] | pH and temperature not specified in the publication | Hedera helix | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oleoyl-[acyl-carrier protein] + reduced acceptor + O2 | Hedera helix | - |
(4Z,9Z)-octadec-4,9-dienoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
| palmitoleoyl-[acyl-carrier protein] + reduced acceptor + O2 | Hedera helix | - |
(4Z,9Z)-hexadec-4,9-dienoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
| palmitoyl-[acyl-carrier protein] + reduced acceptor + O2 | Hedera helix | - |
(4Z)-hexadec-4-enoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
| stearoyl-[acyl-carrier protein] + reduced acceptor + O2 | Hedera helix | desaturation occurs at the DELTA9 position | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hedera helix | - |
English ivy | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Poros 20 CM column chromatography and G-300SW gel filtration | Hedera helix |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Hedera helix | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oleoyl-[acyl-carrier protein] + reduced acceptor + O2 | - |
Hedera helix | (4Z,9Z)-octadec-4,9-dienoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
| palmitoleoyl-[acyl-carrier protein] + reduced acceptor + O2 | - |
Hedera helix | (4Z,9Z)-hexadec-4,9-dienoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
| palmitoyl-[acyl-carrier protein] + reduced acceptor + O2 | - |
Hedera helix | (4Z)-hexadec-4-enoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
| stearoyl-[acyl-carrier protein] + reduced acceptor + O2 | desaturation occurs at the DELTA9 position | Hedera helix | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DELTA4-16:0-ACP desaturase | - |
Hedera helix |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
oleoyl-[acyl-carrier protein] | pH and temperature not specified in the publication | Hedera helix | |
| 0.048 | - |
palmitoleoyl-[acyl-carrier protein] | pH and temperature not specified in the publication | Hedera helix | |
| 0.17 | - |
stearoyl-[acyl-carrier protein] | pH and temperature not specified in the publication | Hedera helix | |
| 0.25 | - |
palmitoyl-[acyl-carrier protein] | pH and temperature not specified in the publication | Hedera helix | |
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