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Literature summary for 1.14.19.2 extracted from

  • Dyer, D.H.; Lyle, K.S.; Rayment, I.; Fox, B.G.
    X-ray structure of putative acyl-ACP desaturase DesA2 from Mycobacterium tuberculosis H37Rv (2005), Protein Sci., 14, 1508-1517.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.19.2

Cloned(Commentary)

Cloned (Comment) Organism
DesA1 overexpressed in Escherichia coli BL21-(DE3) with the natural N-terminal methionine residue as the first amino acid Mycobacterium tuberculosis
DesA2 overexpressed in Escherichia coli BL21-(DE3) with the natural N-terminal methionine residue as the first amino acid Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
DesA2 crystals grown by the method of hanging drop vapor diffusion, one metal bound per subunit which is likely Mn2+, native and selenomethionine-substituted, 2.0 A resolution X-ray structure Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information absence of iron in both the native and selenomethionine-substituted crystals, X-ray fluorescence scan Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 unlabeled, purified DesA2 protein, MALDI-TOF, 31138 and 31345 m/z Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WNZ5 H37Rv,variant DesA2
-
Mycobacterium tuberculosis P9WNZ7 H37Rv, variants DesA1
-
Mycobacterium tuberculosis H37Rv P9WNZ5 H37Rv,variant DesA2
-
Mycobacterium tuberculosis H37Rv P9WNZ7 H37Rv, variants DesA1
-

Purification (Commentary)

Purification (Comment) Organism
DesA2 isoform purified by ion exchange and gel filtration chromatographies Mycobacterium tuberculosis

Subunits

Subunits Comment Organism
dimer DesA2 isoform, gel filtration Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
acyl-ACP-desaturase
-
 Mycobacterium tuberculosis
DesA1
-
 Mycobacterium tuberculosis
DesA2
-
 Mycobacterium tuberculosis